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US 6,147,189 AGranted
Polypeptides that include conformation-constraining groups which flank a protein-protein interaction site
AssigneeVirginia Commonwealth University
InventorsHerbert J. Evans, R. Manjunatha Kini
Life of this patent
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Abstract
Homologs and analogs of naturally-occurring polypeptides contain one or more interaction sites of the natural counterpart. The interaction sites are flanked by conformation-constraining moieties, such as proline or cysteine.
Claims10
A method of producing a conformationally-constrained polypeptide that mimics the activity of an RGD-containing peptide, wherein the method comprises:
- synthesizing the conformationally-constrained polypeptide, wherein the conformationally-constrained polypeptide contains a RGD interaction site of said RGD-containing peptide adjacently flanked on both termini by proline residues in a manner distinct from the manner found in the RGD-containing peptide, wherein the conformationally-constrained polypeptide contains an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8 and SEQ ID NO:9.
A method of producing a conformationally-constrained polypeptide that mimics the activity of an RGD-containing peptide, wherein the method comprises:
- synthesizing the conformationally-constrained polypeptide, wherein the conformationally-constrained polypeptide (i) has a different length than said RGD-containing peptide and (ii) contains an RGD site, wherein the conformationally-constrained polypeptide contains an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8 and SEQ ID NO:9.
Dependencies are inferred from the claim text.