Method for determination of water treatment polymers
AssigneeStrategic Diagnostics Inc.
InventorsPauline Weatherbury, William H. Stemson
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Abstract
A method for determining the presence and/or concentration of a water treatment polymer in an aqueous sample, comprising producing a polyclonal or monoclonal antibody to the water treatment polymer, and using the antibody so produced as a reagent in an immunoassay, conducted on the aqueous sample.
Claims7
A hybridoma which produces antibodies which bind to a polydisperse water treatment polymer, which antibodies can specifically bind with the polymer, the polymer being a phosphinocarboxylic acid of the formula I:
- ##STR4## or a salt thereof in which R" is hydrogen, methyl or ethyl;
- R is hydrogen, C.sub.1 -C.sub.18 alkyl, C.sub.5 -C.sub.12 cycloalkyl, aryl, aralkyl, a residue of the formula:
- ##STR5## in which R" has is previous significance and the sum of m and n is an integer of at most 100, or a residue --OX in which X is hydrogen or Cl-C.sub.4 alkyl;
- and R' is a residue --OX in which X has its previous significance.
A composition useful for determining the presence and/or concentration of a polydisperse water treatment polymer in an aqueous sample, comprising a monoclonal or polyclonal antibody which binds to the water treatment polymer in an immunogenically acceptable carrier;
- wherein the polymer is a phosphinocarboxylic acid of the formula I:
- ##STR6## or a salt thereof in which R" is hydrogen, methyl or ethyl;
- R is hydrogen, C.sub.1 C.sub.18 alkyl, C.sub.5 -C.sub.12 cycloalkyl, aryl, aralkyl, a residue of the formula:
- ##STR7## in which R" has is previous significance and the sum of m and n is an integer of at most 100, or a residue --OX in which X is hydrogen or C.sub.1 -C.sub.4 alkyl and R' is a residue --X in which X has its previous significance.
An immunoassay method for determining the presence and/or concentration of a polydisperse water treatment polymer in an aqueous sample, comprising (a) contacting a polyclonal or monoclonal antibody which binds to the water treatment polymer with the aqueous sample;
- (b) incubating the sample with the antibody for a time effective for the antibody to bind to the water treatment polymer to form a complex;
- and (c) detecting the complex and thereby determining the presence and/or concentration of the water treatment polymer in the aqueous sample;
- wherein the water treatment polymer is a phosphinocarboxylic acid of the formula I:
- ##STR8## or a salt thereof in which R" is hydrogen, methyl or ethyl;
- R is hydrogen, C.sub.1 -C.sub.18 alkyl, C.sub.5 -C.sub.12 cycloalkyl, aryl, aralkyl, a residue of the formula:
- ##STR9## in which R" has is previous significance and the sum of m and n is an integer of at most 100, or a residue --OX in which X is hydrogen or C.sub.1 -C.sub.4 alkyl;
- and R' is a residue --OX in which X has its previous significance.
Dependencies are inferred from the claim text.