Height referencing biochemical cassette
AssigneeIowa State University Research Foundation, Inc.
InventorsMarc D. Porter, Vivian W. Jones, Curtis L. Mosher, Eric Henderson
Life of this patent
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Abstract
A height referencing biochemical cassette comprising of a surface suitable to act as a coupling agent, a height referencing indicator, and molecules bonded to the surface. This cassette is a capable of use to test for bonding to these molecules through measuring the height difference between the indicator and the surface. This invention provides an efficient means to quickly and easily test for bonding of molecules to other molecules.
Claims26
A biocassette, said biocassette providing an internal reference plane for the detection of topographic changes, formed by the process of:
- fabricating a surface that can be made smooth;
- patterning the surface to form a periodic array of coated surfaces and uncoated surfaces, whereby a hydrophobic monolayer is confined on the coated surfaces, said monolayer serving as a height referencing indicator;
- applying a coupling agent to the patterned surface to form an adlayer of coupling agent on the uncoated surfaces, said coupling agent being capable of immobilizing proteinaceous materials or nucleic acids;
- and
- immobilizing at least one protein or nucleic acid on the adlayer.
A method of making a biocassette, said biocassette providing an internal reference plane for the detection of topographic changes, comprising:
- fabricating a surface that can be made smooth;
- patterning the surface to form a periodic array of coated surfaces and uncoated surfaces, whereby a hydrophobic monolayer is confined on the coated surfaces, said monolayer serving as a height referencing indicator;
- applying a coupling agent to the patterned surface to form an adlayer of coupling agent on the uncoated surfaces, said coupling agent being capable of immobilizing proteinaceous materials or nucleic acids;
- and
- immobilizing at least one protein or nucleic acid on the adlayer.
A biocassette, said biocassette providing an internal reference plane for the detection of topographic changes, comprising:
- a surface patterned to form a periodic array of coated surfaces and uncoated surfaces, whereby a hydrophobic monolayer is confined on the coated surfaces, said monolayer serving as a height referencing indicator;
- an adlayer of coupling agent on the uncoated surfaces, said coupling agent being capable of immobilizing proteinaceous materials or nucleic acids;
- and
- at least one immobilized protein or nucleic acid on the adlayer.
A biocassette, said biocassette providing an internal reference plane for the detection of topographic changes, comprising:
- a surface patterned to form a periodic array of coated surfaces and uncoated surfaces, whereby an ODT monolayer is confined on the coated surfaces, said monolayer serving as a height referencing indicator;
- an adlayer of DSU on the uncoated surfaces;
- and
- at least one immobilized protein or nucleic acid on the adlayer.
A method of making a biocassette, said biocassette providing an internal reference plane for the detection of topographic changes, comprising:
- fabricating a surface that can be made smooth;
- patterning the surface to form a periodic array of coated surfaces and uncoated surfaces, whereby an ODT monolayer is confined on the coated surfaces, said monolayer serving as a height referencing indicator;
- applying DSU to the patterned surface to form an adlayer of coupling agent on the uncoated surfaces, said coupling agent being capable of immobilizing proteinaceous materials or nucleic acids;
- and
- immersing the patterned surface with the adlayer of coupling agent into a buffer solution comprising a protein or nucleic acid and a surfactant.
Dependencies are inferred from the claim text.