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US 6,146,891 AGranted
Methods for cultivating cells and propagating viruses
AssigneeSchering Corporation
InventorsRussell G.G. Condon, Nancy V. Connelly, Andreas Frei, Edward Glowacki, Vijay Yabannavar, Serge Batandolo
Life of this patent
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Abstract
This invention relates to methods for the cultivating cells, and in particular to methods for propagating viruses.
Claims24
A method for cultivating cells, comprising:
- (a) cultivating the cells on a first batch of microcarriers until the cells are substantially confluent;
- (b) detaching the cells from the microcarriers without removing the microcarriers from suspension and without allowing the microcarriers to settle;
- (c) after step (b), adding a second batch of microcarriers;
- and
- (d) cultivating the cells further.
2 more elements
Dependencies are inferred from the claim text.