Cyclic lipopeptide acylase
AssigneeFukisawa Pharmaceutical Co., Ltd.
InventorsSatoshi Ueda, Miho Tanaka, Masami Ezaki, Kazutoshi Sakamoto, Seiji Hashimoto, Nobutaka Oohata, Masaru Tsuboi, Michio Yamashita
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Abstract
The present invention provides a cyclic lipopeptide acylase which may effectively deacylate the acyl side chain of a cyclic lipopeptide compound, specifically FR901379 Substance or its analog thereof shown by the following general formula [I], and a process for production of a cyclic peptide compound which comprises the use of said acylase. ##STR1##
Claims12
An isolated acylase enzyme, wherein the acylase enzyme obtained from Oidiodendron sp. No. 30084 (FERM BP-5943), wherein the acylase catalyzes deacylation of the acyl group R.sup.1 of a cyclic lipopeptide compound of the following general formula (I):
- ##STR6## wherein R.sup.1 is acyl;
- R.sup.2 is hydroxy or acyloxy;
- R.sup.3 is hydrogen or hydroxy;
- R.sup.4 is hydrogen or hydroxy;
- R.sup.5 is hydrogen or hyroxysulfonyloxy;
- and
- R.sup.6 is hydrogen or carbamoyl
- or a salt thereof, to give a cyclic peptide of the following general formula (II):
- ##STR7## wherein R.sup.2, R.sup.3, R.sup.4, R.sup.5, and R.sup.6 are the same groups as respectively defined above or a salt thereof:
- wherein the optimum pH for deacylation is from 2 to 4:
- the optimum temperature for deacylation is from 25 to 45.degree. C.;
- the enzyme activity is activated in a concentration dependent manner in the presence of methanol up to 20% by volume in the reaction mixture and the enzyme activity is inhibited at concentrations of methanol over 40% by volume.
Dependencies are inferred from the claim text.