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US 6,146,868 AGranted
Glucuronoxylomannan (GXM)-O-acetylhydrolase of cryptococcus neoformans and uses thereof
AssigneeResearch Development Foundation
InventorsThomas R. Kozel, Sherri L. Bloomer, Anne C. Savoy
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Abstract
The present invention provides a novel enzyme which de-O-acetylates glucuronoxylomannan of Cryptococcus neoformans and a gene encoding such enzyme. Also provided are applications of such enzyme in treating cryptococcosis.
Claims9
DNA encoding glucuronoxylomannan-O-acetylhydrolase (GXM-O-acetylhydrolase), wherein said DNA is selected from the group consisting of:
- (a) isolated DNA which encodes glucuronoxylomannan-O-acetylhydrolase;
- (b) isolated DNA which hybridizes to isolated DNA of (a) above and which encodes glucuronoxylomannan-O-acetylhydrolase;
- and
- (c) isolated DNA differing from the isolated DNAs of (a) and (b) above in codon sequence due to the degeneracy of the genetic code, and which encodes glucuronoxylomannan-O-acetylhydrolase.
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A method of producing a recombinant GXM-O-acetylhydrolase having an amino acid sequence shown in SEQ ID NO:
- 31 comprising the steps of:
- obtaining a vector that comprises an expression region comprising a sequence encoding the amino acid sequence shown in SEQ ID No. 31 operatively linked to a promoter;
- transfecting said vector into a cell;
- and
- culturing said cell under conditions effective for expression of said expression region.
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Dependencies are inferred from the claim text.