US 6,146,867 A · Methods for expressing a maize acetyl CoA carboxylase gene in host cells and encoded protein produced thereby

Abstract

The present invention provides the complete cDNA sequence of maize acetyl CoA carboxylase and a method introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. The method includes the steps of introducing an expression cassette encoding a plant acetyl CoA carboxylase or an antisense DNA sequence complementary to the sequence for a plant acetyl CoA carboxylase gene operably linked to a promoter functional in plant cells, into the cells of a plant tissue and expressing the plant acetyl CoA carboxylase gene. The expression cassette can also be introduced into other host cells to increase yield of a plant acetyl CoA carboxylase crystallized enzyme.

Claims21

Dependent claims

A method of producing a maize acetyl CoA carboxylase comprising:

(a) introducing a recombinant DNA molecule encoding a maize acetyl CoA carboxylase into a population of host cells so as to yield maize acetyl CoA carboxylase, wherein the maize acetyl CoA carboxylase comprises SEQ ID NO:6 or an enzymatically active fragment of SEQ ID NO:6;
and
(b) recovering said maize acetyl CoA carboxylase from said cells.

Dependencies are inferred from the claim text.

Facts

Application

08/695,651Filed 12 Aug 1996 · division

Priority

5 Feb 1993

Granted

14 Nov 2000

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12N 9/93
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: ligases
C12N 15/8247
Involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine: involving modified lipid metabolism, e.g. seed…
C12N 15/8274
For stress resistance, e.g. heavy metal resistance: for herbicide resistance
C12N
Microorganisms or enzymes

Methods for expressing a maize acetyl CoA carboxylase gene in host cells and encoded protein produced thereby

Life of this patent

Methods for expressing a maize acetyl CoA carboxylase gene in host cells and encoded protein produced thereby

Life of this patent

Abstract

Claims21