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AssigneeE.I. du Pont de Nemours and Company
InventorsPaul Veikko Viitanen, Douglas Brian Jordan, Karen Onley Bacot
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Through function complementation of E. coli auxotrophs, the ultimate and pentultimate enzymes of the spinach riboflavin biosynthetic pathway have been cloned, namely, lumazine synthase (LS) and riboflavin synthase (RS). This invention relates to the isolation of nucleic acid fragments from plants or fungi that encode LS protein. The invention also relates to the isolation of nucleic acid fragments from plants or fungi that encode RS protein. In addition, the invention also relates to the construction of chimeric genes encoding all of a portion of LS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant LS in a transformed host cell. Furthermore, the invention also relates to the construction of chimeric genes encoding all of a portion of RS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant or fingal RS in a transformed host cell. In vivo and in vitro methods to identify herbicide or fungicide candidates are included that evaluate the ability of a chemical compound to inhibit the activity of a plant or fungal LS enzyme or a plant or fungal RS enzyme.
An isolated nucleic acid fragment selected from the group consisting of:
A nucleic acid fragment encoding an enzymatically active plant riboflavin synthase enzyme obtained by the method comprising:
A nucleic acid fragment encoding an enzymatically active plant riboflavin synthase enzyme obtained by the method comprising:
Dependencies are inferred from the claim text.