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AssigneeLaboratoires Serobiologiques (Societe Anonyme)
InventorsGilles Pauly, Marc Pauly, Jean-Marc Engasser, Mohamed Ghoul
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A process is provided for the production of glycogens or an extract rich in glycogens from yeast cells, and a cosmetic composition containing them. A given quantity of yeast cells, from a specific culture or recovered as residues of a fermentation process, is subjected to an operation of enrichment in intracellular glycogens by culturing in two phases in the presence of a carbon source. The metabolism of the yeast cells is then stopped. The membranes of the yeast cells are then at least partially disintegrated to free intracellular substances, and the freed intracellular substances are subjected to at least one precipation to precipitate glycogens. A cosmetic composition comprising the glycogens is formulated in admixture with a dermatologically acceptable excipient.
Process for the production of glycogens from viable yeast cells, comprising subjecting said viable yeast cells to an operation of enrichment in intracellular glycogens comprising in a first phase culturing the viable yeast cells in a nutrient medium comprising a source of carbon, nitrogen, phosphorus, mineral elements and vitamins, and in a second maturation phase continuing culturing of said yeast cells in a nutrient medium containing at least one carbon source at a concentration comprised between 1 and 700 g per liter of nutrient medium, and a source of nitrogen such that the viable yeast cells transform the carbon source into intracellular glycogens, stopping metabolism of said viable yeast cells, disintegrating at least partially the membranes of said viable yeast cells to free soluble intracellular substances, and subjecting the freed soluble intracellular substances to at least one precipitation to precipitate glycogens and recovering said glycogens.
Dependencies are inferred from the claim text.