Method for the detection of viable Cryptosporidium parvum oocysts
AssigneesMacquarie Research Ltd., Australian Water Technologies Pty. Ltd.
InventorsKeith Leslie Williams, Graham Vesey, Duncan Veal, Nicholas John Ashbolt, Matthias Dorsch
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Abstract
Oligonucleotide molecules and methods are disclosed for the detection of viable oocysts or other cells of the protozoa species, Cyrptosporidium parvum. Preferred oligonucleotide molecules are selected from the group comprising oligonucleotides having one or more of the following sequences: (a) ACA ATT ATT, (b) CTT TTT GGT, (c) ATT TTA TAT AAA ATA TTT TGA TGA A, (d) TTT TTT TTT TTA GTA T, (e) TAT ATT TTT TAT CTG, (f) CTT TAC TTA CAT GGA TAA CCG, or comprising a part of the sequences (a) to (f) above so as to allow specific hybridization to unique 18S rRNA sequences of C. parvum.
Claims11
An oligonucleotide molecule for the detection of viable oocysts of Cryptosporidium parvum (C. parvum) in an environmental sample to be tested for safety of human use, wherein said molecule specifically hybridizes to a molecule having a DNA sequence of C. parvum, said sequence selected from the group consisting of:
- (a) AAT TTA TAT AAA ATA TTT TGA TGA A (SEQ ID NO:
- 1);
- (b) TTT TTT TTT TTA GTA T (SEQ ID NO:
- 2);
- (c) TAT ATT TTT TAT CTG (SEQ ID NO:
- 3);
- (d) CTT TAC TTA CAT QGA TAA CCG (SEQ ID NO:
- 4);
- (e) ACA ATT AAT (SEQ ID NO:
- 5);
- and
- (f) CTT TTT GGA (SFQ ID NO:
- 6);
- wherein the molecule allows detection of viable oocysts of C. parvum.
Dependencies are inferred from the claim text.