Bacterial expression system
AssigneeThe Hong Kong University of Science and Technology
InventorsWan Keung Wong, Kat Hon Lam
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Abstract
The present invention provides endonuclease-protected vegI promoters, constructs comprising same, bacteria transformed with same, expression products of said constructs produced by bacteria transformed with same, and methods of manufacturing said expression products using said transformed bacteria. Also provided are E. coli transformed with constructs having the vegI promoter, expression products of same and methods of manufacture of said expression products using said transformed E. coli.
Claims26
An endonuclease-protected vegI promoter.
A vegI promoter having a guanine nucleotide substituted at position -11 relative to the transcription start nucleotide beginning at nucleotide +1, said start nucleotide corresponding to nucleotide 51 of SEQ ID NO:
- 12 or SEQ ID NO:
- 13.
A DNA construct for expressing a coding sequence in B. subtilis, comprising operatively linked in the 5' to 3' direction:
- a) an endonuclease-protected vegI promoter;
- b) a DNA coding sequence encoding an expression product;
- and
- c) a 3' non-translated region.
A DNA construct for expressing a coding sequence in B. subtilis, comprising operatively linked in the 5' to 3' direction:
- a) an endonuclease-protected vegI promoter;
- b) at least one cloning site into which may be inserted a DNA coding sequence encoding an expression product;
- and
- c) a 3' non-translated region.
An E. coli bacterium transformed with a DNA construct for expressing a coding sequence, said DNA construct comprising operatively linked in the 5' to 3' direction:
- a) the vegI promoter;
- b) a DNA coding sequence encoding an expression product;
- and
- c) a 3' non-translated region.
Dependencies are inferred from the claim text.