Polynucleotides encoding a sialoadhesin family member-2 (SAF-2)
AssigneesSmithKline Beecham Corporation, The Johns Hopkins University
InventorsKristine Kay Kikly, Connie Lynn Erickson-Miller, Bruce Bochner, Robert Schleimer
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Abstract
SAF-2 polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing SAF-2 polypeptides and polynucleotides in therapy, and diagnostic assays for such.
Claims22
An expression vector comprising a polynucleotide capable of producing a polypeptide comprising the sequence set forth in SEQ ID NO:2 when said expression vector is present in a compatible host cell.
An isolated polynucleotide comprising a polynucleotide sequence that has at least 80% identity to a nucleotide sequence which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2, wherein said polynucleotide sequence may include up to n.sub.n nucleotide alterations over the entire region coding for SEQ ID NO:2, wherein n.sub.n is the maximum number of nucleotide alterations and is calculated by the formula
- n.sub.n .ltoreq.x.sub.n -(x.sub.n .multidot.Y),
- in which x.sub.n is the total number of nucleotides which encode the amino acid sequence of SEQ ID NO:2 and y has a value of 0.80, wherein any non-integer product of x.sub.n and y is rounded down to the nearest integer prior to subtracting such product from x.sub.n.
An isolated polynucleotide comprising a polynucleotide sequence that has at least 80% identity to the nucleotide sequence of SEQ ID NO:1, wherein said polynucleotide sequence may include up to n.sub.n nucleotide alterations over the entire length of SEQ ID NO:1, wherein n.sub.n is the maximum number of nucleotide alterations and is calculated by the formula
- n.sub.n .ltoreq.x.sub.n -(x.sub.n .multidot.Y),
- in which .sub.n is the total number of nucleotides of SEQ ID NO:1 and y has a value of 0.80, wherein any non-integer product of x.sub.n and y is rounded down to the nearest integer prior to subtracting such product from x.sub.n.
An isolated polynucleotide that is fully complementary to an isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2.
An isolated polynucleotide comprising a polynucleotide sequence that has at least 80% identity to a nucleotide sequence which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:4, wherein said polynucleotide sequence may include up to n.sub.n nucleotide alterations over the entire region coding for SEQ ID NO:4, wherein n.sub.n is the maximum number of nucleotide alterations and is calculated by the formula
- n.sub.n .ltoreq.x.sub.n -(x.sub.n .multidot.y),
- in which x.sub.n is the total number of nucleotides which encode the amino acid sequence of SEQ ID NO:4 and y has a value of 0.80, wherein any non-integer product of x.sub.n and y is rounded down to the nearest integer prior to subtracting such product from x.sub.n.
An isolated polynucleotide comprising a polynucleotide sequence that has at least 80% identity to the nucleotide sequence of SEQ ID NO:3, wherein said polynucleotide sequence may include up to n.sub.n nucleotide alterations over the entire length of SEQ ID NO:3, wherein n.sub.n is the maximum number of nucleotide alterations and is calculated by the formula
- n.sub.n .ltoreq.x.sub.n -(x.sub.n .multidot.y),
- in which x.sub.n is the total number of nucleotides of SEQ ID NO:3 and y has a value of 0.80, wherein any non-integer product of x.sub.n and y is rounded down to the nearest integer prior to subtracting such product from x.sub.n.
An isolated polynucleotide that is fully complementary to an isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:4.
Dependencies are inferred from the claim text.