Process for producing hydroxylated cholesterols and dihydroxycholesterols using amycolata
AssigneeMercian Corporation
InventorsKoji Takeda, Tadashi Terasawa, Kazuyuki Dobashi, Takeo Yoshioka
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Abstract
A method for preparing at least one hydroxycholesterol chosen from the group consisting of 25-hydroxycholesterol, 17,25-dihydroxycholesterol and 25,26-dihydroxycholesterol by biological hydroxylation of cholesterol, and the aforementioned dihydroxycholesterols. In the above biological hydroxylation, a microorganism is used which has the abovementioned hydroxylation capacity and which belongs to the genus Amycolata and the genus Sphingomonas.
Claims4
A method for preparing a hydroxylated cholesterol having the formula (II):
- ##STR4## in which R.sup.1 is a hydroxyl radical, and R.sup.2 and R.sup.3 are a hydroxyl radical and a hydrogen atom respectively, or a hydrogen atom and a hydroxyl radical respectively, by biological conversion of cholesterol having the formula (I):
- ##STR5## which method comprises:
- (A) incubating cholesterol having the formula (I) with a strain of Amycolata or a preparation thereof which is able to biologically convert said cholesterol into the hydroxylated cholesterol having the formula (II), in an atmosphere containing oxygen, to produce an incubation-treated solution, in which (1) the hydroxylated cholesterol is either a compound having the formula (II) wherein R.sup.1 and R.sup.2 are a hydroxyl radical, and R.sup.3 is a hydrogen atom, or a compound having the formula (II) wherein R.sup.1 and R.sup.3 are a hydroxyl radical, and R.sup.2 is a hydrogen atom, and the strain of Amycolata is Amycolata saturnea FERM BP-5544, or (2) the hydroxylated cholesterol is a compound having the formula (II) wherein R.sup.1 and R.sup.3 are a hydroxyl radical, and R.sup.2 is a hydrogen atom, and the strain of Amycolata is Amycolata autotrophica ATCC 33796;
- and
- (B) recovering the hydroxylated cholesterol from the incubation-treated solution.
A method for preparing a dihydroxycholesterol having the formula (II-b):
- ##STR6## in which R.sup.2 and R.sup.3 are a hydroxyl radical and a hydrogen atom respectively, or a hydrogen atom and a hydroxyl radical respectively
- by biological conversion of 25-hydroxycholesterol having the formula (II-a):
- ##STR7## which method comprises:
- (A) incubating 25-hydroxycholesterol having the formula (II-a) with Amycolata saturnea FERM BP-5544, or a preparation thereof which is able to biologically convert said 25-hydroxycholesterol into the dihydroxycholesterol having the formula (II-b), in an atmosphere containing oxygen, to produce an incubation-treated solution, and
- (B) recovering the dihydroxycholesterol from the incubation-treated solution.
Dependencies are inferred from the claim text.