Methods for identifying compounds that bind to CSAPK-3 molecules and fragments thereof

A method for identifying a compound which modulates the binding or kinase activity of a naturally occurring allelic variant of apolypeptide consisting of the amino acid sequence of SEQ ID NO:8, wherein the allelic variant is encoded by a nucleic acid molecule which hybridizes to the complement of a nucleic acid molecule consisting of SEQ ID NO:7 or 9, in 6.times.SSC at 45.degree. C., followed by one or more washes in 0.2.times.SSC, 0.1% SDS at 50-65.degree. C., the method comprising:

• a) contacting a cell expressing the allelic variant with a test compound under conditions suitable for modulation of the binding or kinase activity of the allelic variant;
• and
• b) detecting modulation of the binding or kinase activity of the allelic variant by the test compound.

A method for identifying a compound which modulates the kinase activity of a polypeptide comprising at least 100 coniguous amino acids of SEQ ID NO:8, the method comprising:

• a) contacting a cell expressing the polypeptide with a test compound under conditions suitable for modulation of the kinase activity of the polypeptide;
• and
• b) detecting modulation of the kinase activity of the polypeptide by the test compound.

A method for identifying a compound which modulates the binding or kinase activity of a polypeptide comprising the amino acid sequence of SEQ ID NO:8, the method comprising:

• a) contacting the polypeptide with a test compound under conditions suitable for modulation of the binding or kinase activity of the polypeptide;
• and
• b) detecting modulation of the binding or kinase activity of the polypeptide by the test compound.

A method for identifying a compound which modulates the binding or Kinase activity of a polypeptide consisting of the amino acid sequence of SEQ ID NO:8, the method comprising:

• a) contacting the polypeptide with a test compound under conditions suitable for modulation of the activity of the binding or kinase polypepride;
• and
• b) detecting modulation of the activity of the binding or Kinase polypeptide by the test compound.

A method for identifying a compound which modulates the binding or kinase activity of a naturally occurring allelic variant of a polypeptide consisting of the amino acid sequence of SEQ ID NO:8, wherein the allelic variant is encoded by a nucleic acid molecule which hybridizes to the complement of a nucleic acid molecule consisting of SEQ ID NO:7 or 9, in 6.times.SSC at 45.degree. C., followed by one or more washes in 0.2.times.SSC, 0.1% SDS at 50-65.degree. C., the method comprising:

• a) contcting the allelic variant with a test compound under conditions suitable for modulation of the binding or kinase activity of the polypeptide;
• and
• b) detecting modulation of the activity of the binding or kinase allelic variant by the test comapound.

A method for identifying a compound which modulates the kinase activity of a polypeptide comprising at least 100 contiguous amino acids of SEQ ID NO:8, the method comprising:

• a) contacting the polypeptide with a test compound under conditions suitable for modulation of the kinase activity of the polypeptide;
• and
• b) detecting modulation of the kinase activity of the polypeptde by the test compound.