US 6,146,827 A · Rath genes and polypeptides and methods for the treatment and diagnosis of immune disorders

Abstract

The present invention relates, first, to the identification of novel nucleic acid molecules, termed RATH genes and RATH gene products encoded by such nucleic acid molecules, or degenerate variants thereof, that participate in the regulation, control and/or modulation of G-protein-mediated signal transduction involved in T cell activation, including, but not limited to T helper (TH) cell and TH cell subpopulation activation. Specifically, the nucleic acid molecules of the present invention include the genes corresponding to the mammalian RATH genes, including the RATH1.1 genes. Sequence analysis indicates that the RATH genes are novel genes belonging to the RGS ("regulator of G-protein signalling") gene family, a gene family which encodes gene products involved in G-protein-mediated signal transduction.

Claims16

Dependent claims

A method for detecting a RATH nucleic acid molecule in a T helper cell sample comprising contacting a T helper cell sample with a reagent which detects the RATH nucleic acid molecule in the sample, wherein the RATH nucleic acid molecule comprises:

(a) a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, or the amino acid sequence encoded by the cDNA clone of ATCC Accession No. 98116;
(b) a nucleotide sequence that encodes an RGS domain of the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, or the amino acid sequence encoded by the cDNA clone of ATCC Accession No. 98116;
or
(c) a nucleotide sequence that hybridizes to the complement of a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, or the amino acid sequence encoded by the cDNA clone of ATCC Accession No. 98116 under conditions comprising incubation at 65.degree. C. in 0.5 M NaHPO.sub.4, 7% SDS, 1 mM EDTA followed by washing in 0.1.times.SSC/0.1% SDS at 68.degree. C.

1 more element

A method for detecting a genetic alteration of a RATH nucleic acid molecule in a T helper cell sample, comprising detecting the presence of at least one of (i) a point mutation in the RATH nucleic acid molecule;

or (ii) an insertion or deletion in the RATH nucleic acid molecule, wherein the RATH nucleic acid molecule comprises:
(a) a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, or the amino acid sequence encoded by the cDNA clone of ATCC Accession No. 98116;
(b) a nucleotide sequence that encodes an RGS domain of the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, or the amino acid sequence encoded by the cDNA clone of ATCC Accession No. 98116;
or
(c) a nucleotide sequence that hybridizes to the complement of a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, or the amino acid sequence encoded by the cDNA clone of ATCC Accession No. 98116 under conditions comprising incubation at 65.degree. C. in 0.5 M NaHPO.sub.4, 7% SDS, 1 mM EDTA followed by washing in 0.1.times.SSC/0.1% SDS at 68.degree. C.

2 more elements

A method for detecting a genetic alteration of a RATH gene sequence in a T helper cell sample, said method comprising detecting the presence of a chromosomal rearrangement in the RATH gene sequence in the sample, wherein the RATH gene sequence comprises a RATH nucleic acid molecule further comprising:

(a) a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, or the amino acid sequence encoded by the cDNA clone of ATCC Accession No. 98116;
(b) a nucleotide sequence that encodes an RGS domain of the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, or the amino acid sequence encoded by the cDNA clone of ATCC Accession No. 98116;
or
(c) a nucleotide sequence that hybridizes to the complement of a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, or the amino acid sequence encoded by the cDNA clone of ATCC Accession No. 98116 under conditions comprising incubation at 65.degree. C. in 0.5 M NaHPO.sub.4, 7% SDS, 1 mM EDTA followed by washing in 0.1.times.SSC/0.1% SDS at 68.degree. C.

1 more element

Dependencies are inferred from the claim text.

Rath genes and polypeptides and methods for the treatment and diagnosis of immune disorders

Life of this patent

Rath genes and polypeptides and methods for the treatment and diagnosis of immune disorders

Life of this patent

Abstract

Claims16