Avian leukosis virus subgroup J envelope gene product for diagnosis and immunogenic composition
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsLucy F. Lee, Aly M. Fadly, Henry D. Hunt
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Abstract
The envelope (env) gene from avian leukosis virus subgroup J (ALV-J) strain Hc1 has been isolated, sequenced and cloned into an expression vector. The ALV-J Hc1 env gene and expressed protein are useful for development of diagnostic assays to detect Hc1-specific nucleic acid and proteins and for eliciting an immune response in chickens.
Claims8
An isolated nucleic acid molecule selected from the group consisting of RNA, cDNA, and double stranded DNA, wherein said molecule comprises a fragment encoding an envelope protein having the sequence of SEQ ID NO:2 and conservative substitutions thereof having a neutralization epitope such that antibody raised to said protein neutralizes both strains HPRS-103 and ADOL-Hc1 of avian leukosis virus subgroup J.
An isolated nucleic acid molecule consisting of a sequence that is 98% identical to SEQ ID NO:1.
A vector selected from the group consisting of an expression vector and a cloning vector, wherein said vector comprises:
- (1) a DNA molecule encoding an envelope protein having the sequence of SEQ ID NO:2 and conservative substitutions thereof having a neutralization epitope such that antibody raised to said protein neutralizes both strains HPRS-103 and ADOL-Hc1 of avian leukosis virus subgroup J, and
- (2) appropriate regulatory sequences for expression of said protein in a suitable host cell when said vector is an expression vector, or appropriate restriction enzyme sites and marker when said vector is a cloning vector.
Dependencies are inferred from the claim text.