Plant steroid 5.alpha. reductase, DET2
AssigneeThe Salk Institute for Biological Studies
InventorsJoanne Chory, Jianming Li
Life of this patent
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Abstract
A novel plant steroid 5.alpha. reductase, DET2, is provided, as well as polynucleotides encoding DET2. DET2 is useful in promoting increased plant yield and/or increased plant biomass. Genetically modified plants characterized as having increased yield and methods for producing such plants are also provided.
Claims17
An isolated polynucleotide encoding an amino acid sequence as set forth in SEQ ID NO:2.
A method of producing a genetically modified plant characterized as having increased yield as compared to a wild-type plant, said method comprising:
- contacting a plant cell with at least one nucleic acid sequence encoding DET2, said nucleic acid sequence operably associated with a promoter, to obtain a transformed plant cell;
- producing a plant from said transformed plant cell;
- and
- selecting a plant exhibiting said increased yield.
A method of producing a plant characterized as having increased yield, said method comprising:
- contacting a susceptible plant with a DET2 promoter-inducing amount of exogenous brassinolide necessary to elevate DET2 gene expression above DET2 expression in a plant not contacted with the agent.
An isolated polynucleotide selected from the group consisting of:
- a) SEQ ID NO:
- 1;
- b) SEQ ID NO:
- 1, wherein T can also be U;
- c) nucleic sequences complementary to SEQ ID NO:1;
- and
- d) fragments of a), b), or c), that are at least 15 bases in length and that selectively hybridize to DNA which encodes the polypeptide of SEQ ID NO:2.
Dependencies are inferred from the claim text.