Method for transferring gene
AssigneeJapan as represented by Director General of Ministry of Agriculture, Forestry and Fisheries National Institue of Agrobiological Resources
InventorsKenjirou Ozawa, Yasunobu Ohkawa, Teruo Ishige
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Abstract
A method for transferring a long-chain DNA into protoplasts, comprising the steps of: (a) mixing a suspension of protoplasts, polyethylene glycol, and a long-chain DNA to give a final concentration of the DNA of at least 50 .mu.g/ml; (b) collecting, washing, and culturing the protoplasts in a medium; and (c) selecting transformed cells, is provided. By this gene transfer method, long-chain DNA can be conveniently transferred into a wide variety of plant cells.
Claims12
A method for transferring a long-chain DNA into protoplasts, comprising the steps of:
- (a) mixing a suspension of protoplasts, polyethylene glycol, and a long-chain DNA of up to about 108 kb in length to give a final concentration of the DNA of at least 50 .mu.g/ml;
- (b) collecting, washing, and culturing the protoplasts in a medium;
- and
- (c) selecting transformed cells.
A method for transferring a long-chain DNA into protoplasts, comprising the steps of:
- (a) mixing a suspension of protoplasts, polyethylene glycol, and a long-chain DNA of up to about 108 kb in length to give a final concentration of the DNA of at least 100 .mu.g/ml;
- (b) collecting, washing, and culturing the protoplasts in a medium;
- and
- (c) selecting transformed cells.
Dependencies are inferred from the claim text.