Method for separation and synthetic polymers that can be used as separation media in the method
AssigneePharmacia Biotech AB
InventorsJan Ericsson, Eva Berggren, Liselotte Lundh
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Abstract
The present invention is directed towards a separation media based on adsorption comprising a polyvinyl ether having different or identical vinyl ether subunits, and towards an insoluble polyvinyl ether comprising free terminal valencies, side hydrogen or methyl groups, as well as plurality of hydrophillic organic groups, wherein hydrogen is replaced by organic residues having affinity to bioorganic molecules.
Claims28
A poly(vinyl ether) which is insoluble in water or aqueous liquids comprising a chain of repeating vinyl ether subunits and having the structure ##STR11## where a) the free terminal valences bind to groups derived from the termination or initiation of the polymerization reaction used to synthesize the poly(vinyl ether);
- b) X.sub.1, X.sub.2, X.sub.3 . . . X.sub.m and Y.sub.1, Y.sub.2, Y.sub.3 . . . Y.sub.m are hydrogen or methyl;
- c) R.sub.1, R.sub.2 . . . R.sub.m' are groups selected from the group consisting of
- i) hydrocarbyl groups, and
- ii) R.sub.1 =R'.sub.1 --B.sub.1 --, R.sub.2 =R'.sub.2 --B.sub.2 -- . . . R.sub.m' =R'.sub.m' --B.sub.m' -- where R'.sub.1, R'.sub.2 . . . R'.sub.m' in turn are selected from the group consisting of OH, NH.sub.2 and substituted forms thereof, in which a hydrogen has been replaced by an organic residue R", wherein R" is an affinity ligand having affinity to a bioorganic molecule, a thiol function (SH) or a function that quantitatively forms a disulfide with a thiol function, and B.sub.1, B.sub.2 . . . B.sub.m' (=B) are hydrophilic organic bridges, respectively;
- d) m' is an integer giving the number of blocks containing identical repeating vinyl subunits;
- and
- e) n.sub.1, n.sub.2, . . . n.sub.m' are integers greater than zero and n.sub.1 +n.sub.2 + . . . +n.sub.m' =n, where n is the number of vinyl subunits in the chain, with the proviso that a plurality of the vinyl ether subunits carry R.sub.1, R.sub.2 . . . or R.sub.m' that are selected among HO--B-- or H.sub.2 N--B-- or forms thereof in which a hydroxy hydrogen or an amino hydrogen is substituted by a hydrophilic organic group;
- wherein said poly(vinyl ether) is insoluble in water or aqueous liquids by
- a) at least one of said organic residues R" being covalently attached to a support (matrix carrier) insoluble in the aqueous liquid;
- b) a plurality of said organic residues R" participating in inter- and/or intramolecular crosslinking of the poly(vinyl ether);
- or
- c) the presence of a plurality of hydrophobic R groups that are physically adsorbed to a hydrophobic support (matrix carrier).
A poly(vinyl ether) comprising a chain of repeating vinyl ether subunits and having the structure ##STR12## where a) the free terminal valences bind to groups derived from the termination or initiation of the polymerization reaction used to synthesize the poly(vinyl ether);
- b) X.sub.1, X.sub.2, X.sub.3 . . . X.sub.m and Y.sub.1, Y.sub.2, Y.sub.3 . . . Y.sub.m are hydrogen or methyl;
- c) R.sub.1, R.sub.2 . . . R.sub.m' are groups selected from the group consisting of
- i) hydrocarbyl groups, and
- ii) R.sub.1 =R'.sub.1 --B.sub.1 --, R.sub.2 =R'.sub.2 --B.sub.2 -- . . . R.sub.m' =R'.sub.m' --B.sub.m' -- where R'.sub.1, R'.sub.2 . . . R'.sub.m' in turn are selected from the group consisting of OH, NH.sub.2 and substituted forms thereof in which a hydrogen has been replaced by an organic residue R", wherein R" is an affinity ligand having affinity to a bioorganic molecule, a thiol function (SH) or a function that quantitatively forms a disulfide with a thiol function, and B.sub.1, B.sub.2 . . . B.sub.m' (=B) are hydrophilic organic bridges, respectively;
- d) m' is an integer giving the number of blocks containing identical repeating vinyl subunits;
- and
- e) n.sub.1, n.sub.2, . . . n.sub.m' are integers greater than zero and n.sub.1 +n.sub.2 + . . . +n.sub.m' =n, where n is the number of vinyl subunits in the chain, with the proviso that a plurality of the vinyl ether subunits carry R.sub.1, R.sub.2 . . . or R.sub.m' that are selected among HO--B-- or H.sub.2 N--B-- or forms thereof in which a hydroxy hydrogen or an amino hydrogen is substituted by a hydrophilic organic group;
- and
- wherein said poly(vinyl ether) is complexed to a bioorganic molecule selected from substances exhibiting polypeptide structure, carbohydrate structures, lipid structures, steroid structure, oligonucleotide structure, nucleotide structure or amino acid structure.
A poly(vinyl ether) comprising a chain of repeating vinyl ether subunits and having the structure ##STR13## where a) the free terminal valences bind to groups derived from the termination or initiation of the polymerization reaction used to synthesize the poly(vinyl ether);
- b) X.sub.1, X.sub.2, X.sub.3 . . . X.sub.m and Y.sub.1, Y.sub.2, Y.sub.3 . . . Y.sub.m are hydrogen or methyl;
- c) R.sub.1, R.sub.2 . . . R.sub.m' are groups selected from the group consisting of
- i) hydrocarbyl groups, and
- ii) R.sub.1 =R'.sub.1 --B.sub.1 --, R.sub.2 =R'.sub.2 --B.sub.2 -- . . . R.sub.m' =R'.sub.m' --B.sub.m' -- where R'.sub.1, R'.sub.2 . . . R'.sub.m' in turn are selected from the group consisting of OH, NH.sub.2 and substituted forms thereof in which a hydrogen has been replaced by an organic residue R" and B.sub.1, B.sub.2 . . . B.sub.m' (=B) are hydrophilic organic bridges, respectively;
- d) m' is an integer giving the number of blocks containing identical repeating vinyl subunits;
- and
- e) n.sub.1, n.sub.2, . . . n.sub.m' are integers greater than zero and n.sub.1 +n.sub.2 + . . . +n.sub.m' =n, where n is the number of vinyl subunits in the chain, with the proviso that a plurality of the vinyl ether subunits carry R.sub.1, R.sub.2 . . . R.sub.m' that are selected among HO--B-- or H.sub.2 N--B-- or forms thereof in which a hydroxy hydrogen or an amino hydrogen is substituted by a hydrophilic organic group, wherein said poly(vinyl ether) is insoluble in water or aqueous liquids by
- i) at least one of said organic residues R" being covalently attached to a support (matrix carrier) insoluble in the aqueous liquid;
- ii) a plurality of said organic residues R" participating in inter- and/or intramolecular crosslinking of the poly(vinyl ether);
- or
- iii) the presence of a plurality of hydrophobic R groups that are physically adsorbed to a hydrophobic support (matrix carrier).
A media for separation based on adsorption comprising a poly(vinyl ether) comprising a chain of repeating vinyl ether subunits and having the structure ##STR14## where a) the free terminal valences bind to groups derived from the termination or initiation of the polymerization reaction used to synthesize the poly(vinyl ether);
- b) X.sub.1, X.sub.2, X.sub.3 . . . X.sub.m and Y.sub.1, Y.sub.2, Y.sub.3 . . . Y.sub.m are hydrogen or methyl;
- c) R.sub.1, R.sub.2 . . . R.sub.m' are groups selected from the group consisting of
- i) hydrocarbyl groups, and
- ii) R.sub.1 =R'.sub.1 --B.sub.1 --, R.sub.2 =R'.sub.2 --B.sub.2 -- . . . R.sub.m' =R'.sub.m' --B.sub.m' -- where R'.sub.1, R'.sub.2 . . . R'.sub.m' in turn are selected from the group consisting of OH, NH.sub.2 and substituted forms thereof in which a hydrogen has been replaced by an organic residue R" and B.sub.1, B.sub.2 . . . B.sub.m' (=B) are hydrophilic organic bridges, respectively;
- d) m' is an integer giving the number of blocks containing identical repeating vinyl subunits;
- and
- e) n.sub.1, n.sub.2, . . . n.sub.m' are integers greater than zero and n.sub.1 +n.sub.2 + . . . +n.sub.m' =n, where n is the number of vinyl subunits in the chain, with the proviso that a plurality of the vinyl ether subunits carry R.sub.1, R.sub.2 . . . or R.sub.m' that are selected among HO--B-- or H.sub.2 N--B-- or forms thereof in which a hydroxy hydrogen or an amino hydrogen is substituted by a hydrophilic organic group.
An absorption-based separation media comprising a chain of repeating vinyl ether subunits and having the structure ##STR15## where a) the free terminal valences bind to groups derived from the termination or initiation of the polymerization reaction used to synthesize the poly(vinyl ether);
- b) X.sub.1, X.sub.2, X.sub.3 . . . X.sub.m and Y.sub.1, Y.sub.2, Y.sub.3 . . . Y.sub.m are hydrogen or methyl;
- c) R.sub.1, R.sub.2 . . . R.sub.m' are groups selected from the group consisting of
- i) hydrocarbyl groups, and
- ii) R.sub.1 =R'.sub.1 --B.sub.1 --, R.sub.2 =R'.sub.2 --B.sub.2 -- . . . R.sub.m' =R'.sub.m' --B.sub.m' -- where R'.sub.1, R'.sub.2 . . . R'.sub.m' in turn are selected from the group consisting of OH, NH.sub.2 and substituted forms thereof in which a hydrogen has been replaced by an organic residue R" and B.sub.1, B.sub.2 . . . B.sub.m' (=B) are hydrophilic organic bridges, respectively;
- d) m' is an integer giving the number of blocks containing identical repeating vinyl subunits;
- and
- e) n.sub.1, n.sub.2, . . . n.sub.m' are integers greater than zero and n.sub.1 +n.sub.2 + . . . +n.sub.m' =n, where n is the number of vinyl subunits in the chain, with the proviso that a plurality of the vinyl ether subunits carry R.sub.1, R.sub.2 . . . or R.sub.m' that are selected among HO--B-- or H.sub.2 N--B-- or forms thereof in which a hydroxy hydrogen or an amino hydrogen is substituted by a hydrophilic organic group, wherein said poly(vinyl ether) is insoluble in water or aqueous liquids by
- i) at least one of said organic residues R" being covalently attached to a support (matrix carrier) insoluble in the aqueous liquid;
- ii) a plurality of said organic residues R" participating in inter- and/or intramolecular crosslinking of the poly(vinyl ether);
- or
- iii) the presence of a plurality of hydrophobic R groups that are physically adsorbed to a hydrophobic support (matrix carrier).
Dependencies are inferred from the claim text.