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US 6,143,727 AGranted
Specific expression vectors and methods of use
AssigneeBaylor College of Medicine
InventorsDennis R. Roop, Joseph A. Rothnagel, David A. Greenhalgh
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Abstract
Gene therapy by using specific expression vectors within the epidermis or epidermal cells. These vectors incorporate regulatory sequences of tissue and differentiation-specific genes.
Claims13
A method of treating skin cancer in a mammal comprising administering a loricrin or K6 keratin expression vector at or directly around the site of a skin cancer cell, wherein said vector comprises in operable association:
- a nucleic acid sequence encoding a wild-type p53 protein;
- a 5' flanking region from a mammalian gene including necessary sequences for expression of said nucleic acid sequence;
- a 3' flanking region from a mammalian loricrin or a mammalian keratin K6 gene which regulates expression predominantly in epidermal tissue;
- and
- a linker connecting said 5' flanking region to said nucleic acid sequence, said linker having a position in which said nucleic acid sequence is inserted,
- wherein expression of said p53 protein by said skin cancer cell results in inhibition of the cell's proliferation.
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Dependencies are inferred from the claim text.