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US 6,143,577 AGranted
Process and installations for separation of magnetic particles in a fluid for biological analysis, and application of said process
AssigneeBiocom S.A.
InventorJean-Claude Bisconte Sconte De Saint Julien
Life of this patent
Estimating the term…
Abstract
A process for magnetic immunoseparation of cells, in particular bacteria, fetal cells, stock cells of bone marrow and circulating cancerous cells, of the type consisting in affixing the target cells on paramagnetic balls and in causing a magnetic field to act on a sample containing the affixed cells, the free cells and the surplus paramagnetic balls, in order to isolate the paramagnetic balls, in that the sample is caused to circulate in a tube the section of which is much less than the length over which the magnetic field is applied.
Claims14
A process for separating cells, comprising:
- contacting a sample containing (1) target cells which are affixed to paramagnetic balls, (2) free cells, and (3) surplus paramagnetic balls, with a nonhomogeneous magnetic field,
- wherein, during the contacting, the sample is circulated in a tube, wherein the nonhomogeneous magnetic field is applied over a section of the tube measuring less than the length of the tube, and wherein the tube has a proximal portion and a distal portion with respect to fluid flow in the tube,
- wherein the surplus paramagnetic balls are immobilized in the proximal portion of the tube and the target cells which are affixed to paramagnetic balls are immobilized in the distal portion of the tube.
Dependencies are inferred from the claim text.