US 6,143,564 A · Use of the polar body chromosomes for the production of embryos and normal offspring

Abstract

The invention provides a method for obtaining a live offspring having maternal chromosomes derived from a live first polar body of an oocyte that has completed the first meiotic division. It has been discovered herein that chromosomes in the first polar body are able to participate in normal embryonic development if they are allowed to complete the second meiotic division within an enucleated mature oocyte, and are then allowed to mingle with chromosomes of a spermatozoon. The invention further provides a method for producing up to four embyros or live offspring having the chromosomes of a single oocyte, by using both the first polar body chromosomes, and the second polar body chromosomes to reconstitute recipient enucleated (fertilized) oocytes.

Claims15

Dependent claims

A method for obtaining an embryo having chromosomes derived from a first polar body, comprising the steps of:

collecting a live first polar body from a first oocyte;
introducing at least a portion of the first polar body that includes all the chromosomes into an enucleated recipient oocyte to form a chromosomally reconstituted recipient oocyte;
fertilizing the reconstituted recipient oocyte;
and
allowing the fertilized reconstituted recipient oocyte to develop into an embryo.

2 more elements

Dependencies are inferred from the claim text.

Use of the polar body chromosomes for the production of embryos and normal offspring

Life of this patent

Use of the polar body chromosomes for the production of embryos and normal offspring

Life of this patent

Abstract

Claims15