Back to results
US 6,143,561 AGranted
DNA encoding plastid pyruvate dehydrogenase and branched chain oxoacid dehydrogenase components
AssigneeThe Curators of the University of Missouri
InventorsDouglas D. Randall, Brian P. Mooney, Mark L. Johnston, Michael H. Luethy, Jan A. Miernyk
Life of this patent
Estimating the term…
Abstract
Provided are nucleic acid sequences encoding E1.alpha., E1.beta., and E2 subunits of plastid pyruvate dehydrogenase complexes and branched chain oxoacid dehydrogenase complexes.
Claims18
An isolated DNA molecule, comprising a nuclcotide sequence selected from the group consisting of:
- (a) the nuclcotide sequence shown in SEQ ID NO:1, or the complement thereof;
- (b) a nucleotide sequence that hybridizes to said nuccotide sequence of (a) under a wash stringency equivalent to 0.1.times.SSC, 0.1% SDS, at 55.degree. C., and which encodes a polypeptide having enzymatic activity differing from that of Arabidopsis thaliana plastid pyruvate dehydrogenase complex E1.alpha. subunit by about 30% or less;
- (c) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (a), but which is degenerate in accordance with the degeneracy of the genetic code;
- and
- (d) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (b), but which is degenerate in accordance with the degeneracy of the genetic code.
2 more elements
An isolated DNA molecule, comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence shown in SEQ ID NO:3, or the complement thereof;
- (b) a nucleotide sequence that hybridizes to said nucleotide sequence of (a) under a wash stringency equivalent to 0.1.times.SSC, 0.1% SDS, at 55.degree. C., and which encodes a polypeptide having enzymatic activity differing from that of Arabidopsis thaliana plastid pyruvate dehydrogenase complex E1.beta. submit by about 30% or less;
- (c) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (a), but which is degenerate in accordance with the degeneracy of the genetic code;
- and
- (d) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (b), but which is degenerate in accordance with the degeneracy of the genetic code.
2 more elements
An isolated DNA molecule, comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence shown in SEQ ID NO:5, or the complement thereof;
- (b) a nucleotide sequence that hybridizes to said nucleotide sequence of (a) under a wash stringency equivalent to 0.1.times.SSC, 0.1% SDS, at 55.degree. C., and which encodes a polypeptidc having enzymatic activity differing from of Arabidopsis thaliana plastid pyruvate dehydrogenase complex E2 component by about 30% or less;
- (c) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (a), but which is degenerate in accordance with the degeneracy of the genetic code;
- and
- (d) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (b), but which is degenerate in accordance with the degeneracy of the genetic code.
2 more elements
An isolated DNA molecule, comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence shown in SEQ ID NO:11, or the complement thereof;
- (b) a nucleotide sequence that hybridizes to said nucleotide sequence of (a) under a wash stringency equivalent to 0.1.times.SSC, 0.1% SDS, at 55.degree. C., and which encodes a polypeptide having enzymatic activity differing from that of Arabidopsis thaliana branched chain 2-oxoacid dehydrogenase complex E1.alpha. subunit by about 30% or less;
- (c) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (a), but which is degenerate in accordance with the degeneracy of the genetic code;
- and
- (d) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (b), but which is degenerate in accordance with the degeneracy of the genetic code.
2 more elements
An isolated DNA molecule, comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence shown in SEQ ID NO:13, or the complement thereof;
- (b) a nucleotide sequence that hybridizes to said nucleotide sequence of (a) under a wash stringency equivalent to 0.1.times.SSC, 0.1% SDS, at 55.degree. C., and which encodes a polypeptide having enzymatic activity differing from that of Arabidopsis thaliana branched chain 2-oxoacid dehydrogenase complex E1.beta. subunit by about 30% or less;
- (c) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (a), but which is degenerate in accordance with the degeneracy of the genetic code;
- and
- (d) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (b), but which is degenerate in accordance with the degeneracy of the genetic code.
2 more elements
An isolated DNA molecule, comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence shown in SEQ ID NO:15, or the complement thereof;
- (b) a nucleotide sequence that hybridizes to said nucleotide sequence of (a) under a wash stringency equivalent to 0.1.times.SSC, 0.1% SDS, at 55.degree. C., and which encodes a polypeptide having enzymatic activity differing from that of Arabidopsis thaliana branched chain 2-oxoacid dehydrogenase complex E2 component by about 30% or less;
- (c) a nuclcotide sequence encoding the same amino acid sequence as said nuclcotide sequence of (a), but which is degenerate in accordance with the degeneracy of the genetic code;
- and
- (d) a nuelcotide sequence encoding the same amino acid sequence as said nucleotide sequence of (b), but which is degenerate in accordance with the degeneracy of the genetic code.
2 more elements
Dependencies are inferred from the claim text.