Methods for the production of chicken monoclonal antibodies
AssigneeArch Development Corporation
InventorsNancy M. Michael, Mary Ann Accavitti, Craig B. Thompson
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Abstract
The present invention provides methods for producing antibodies against a variety of antigens, including mammalian antigens with highly conserved epitopes. In addition, the present invention provides improved methods and compositions for the cloning and manipulation of immunoglobulin genes as well as antibodies derived therefrom.
Claims17
An isolated antibody produced by a process comprising the steps of:
- (i) immunizing a chicken with an antigen composition;
- (ii) isolating B cells from said chicken;
- (iii) immortalizing said B cells;
- (iv) selecting an immortalized antibody producing B cell and preparing nucleic acids encoding the antigen binding regions of the light and heavy chain genes thereof;
- (v) cloning said heavy and light chain antigen binding regions, respectively, into vectors encoding
- (a) the constant and leader regions of a mammalian immunoglobulin heavy chain,
- (b) the constant and leader regions of a mammalian immunogloblin light chain;
- (vi) transferring said vectors into a suitable host cell;
- (v) culturing said host cell;
- and
- (vi) isolating said antibody.
An isolated chicken chimeric antibody that binds immunologically to an antigen from the group consisting of CFTR, transforming growth factor beta, transcription factors DNA binding molecules;
- cyclin dependent kinases and RNA binding proteins, wherein said antibody comprises (I) chicken antibody variable sequences and (ii) antibody constant and leader sequences from a mammal.
An isolated antibody that binds immunologically to an antigen selected from the group consisting of lipids, phospholipids and carbohydrates, wherein said antibody comprises (i) chicken antibody variable sequences and (ii) antibody constant and leader sequences from a mammal.
An isolated cell that produces an antibody that binds immunologically to an antigen selected from the group consisting of lipids, phospholipids, and carbohydrates, wherein said antibody comprises (i) chicken antibody variable sequences and (ii) antibody constant and leader sequences from a mammal.
Dependencies are inferred from the claim text.