Delivery of polypeptide-encoding plasmid DNA into the cytosol of macrophages by attenuated listeria suicide bacteria
AssigneeSchering Corporation
InventorWerner Goebel
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Abstract
The invention relates to the introduction of DNA or RNA sequences into a mammalian cell to achieve controlled expression of a polypeptide. It is therefore useful in gene therapy, vaccination, and any therapeutic situation in which a polypeptide should be administered to a host or cells of said host, as well as for the production of polypeptides by mammalian cells, e.g., in culture or in transgenic animals.
Claims14
An attenuated invasive intracellular Listeria bacterium capable of infecting a mammalian host or host cell thereof, but having a decreased ability in intra- and intercellular movement in said host as compared to a wild type bacterium, transformed with
- (a) a promoter activated when said bacterium is present in the cytosol of a host cell, operably linked to a structural gene or a biologically active fragment thereof encoding a Listeria bacteriophage lysin which is lethal to the bacterium;
- (b) a host cell-compatible promoter, operably linked to a structural gene or fragment thereof, encoding a polypeptide.
A plasmid comprising (a) a promoter activated when it is present in an invasive Listeria Bacterium which is in the cytosol of a mammalian host cell, operably linked to a structural gene encoding a Listeria bacteriophage Lysin which is lethal to the bacterium.
Dependencies are inferred from the claim text.