Method for reducing phosphorus content of edible oils
AssigneeNovo Nordisk A/S
InventorsIb Groth Clausen, Shamkant Anant Patkar, Kim Borch, Martin Barfoed, Kim Clausen, Claus Crone Fuglsang, Lone Dybdal, Torben Halkier
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Abstract
The present invention relates to a method for reducing the content of phosphorous containing components in an edible oil comprising a high amount of non-hydratable phosphorus content, wherein said method comprises use of a phospholipase. Further the present invention relates to an enzyme with phospholipase activity, a cloned DNA sequence encoding the enzyme with phospholipase activity, a method of producing the enzyme, and the use of said enzyme for a number of industrial applications.
Claims10
An isolated nucleic acid comprising a sequence selected from the group consisting of:
- (a) the phospholipase A-encoding sequence cloned into plasmid pYES 2.0 present in Escherichia coli DSM 11299;
- (b) nucleotides 23-1063 of SEQ ID NO:1
- (c) nucleotides 113-1063 of SEQ ID NO:1;
- (d) nucleotides 113-931 of SEQ ID NO:1;
- (e) a nucleic acid encoding amino acids 31-346 of SEQ ID NO 2;
- (f) a nucleic acid encoding amino acids 31-303 of SEQ ID NO 2;
- and
- (g) a nucleic acid which is at least 70% homologous with any of the foregoing nucleic acids, wherein said nucleic acid encodes a polypeptide exhibiting phospholipase A activity.
Dependencies are inferred from the claim text.