DNA encoding a thermostable DNA polymerase
AssigneeToyo Boseki Kabushiki Kaisha
InventorsTadayuki Imanaka, Masahiro Takagi, Masaaki Morikawa
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Abstract
A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3'-5' exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.
Claims7
A recombinant DNA expression vector that comprises a foreign DNA sequence inserted into a vector, wherein the foreign sequence encodes the thermostable DNA polymerase of SEQ ID NO. 1 derived from a KOD 1 strain which belongs to hyperthermophilic archaeon.
A method for producing a DNA polymerase obtainable from a KOD 1 strain belonging to Hyperthromophilic archaeon following the steps of:
- (a) inserting a DNA sequence encoding a thermostable DNA polymerase of a KOD1 strain into an expression vector thereby producing a DNA recombinant expression vector;
- (b) transforming host cells with said DNA recombinant expression vector;
- (c) culturing transformed host cells obtained in step (b) in a suitable media to provide for expression of said thermostable DNA polymerase;
- and
- (d) recovering expressed thermostable DNA polymerase of SEQ ID NO. 1 from said cultured transformed host cells.
A method for producing a thermostable DNA polymerase following the steps of:
- (a) inserting a DNA sequence of SEQ ID NO. 5 encoding a thermostable DNA polymerase of a KOD1 strain into an expression vector thereby producing a DNA recombinant expression vector;
- (b) transforming host cells with said DNA recombinant expression vector;
- (c) culturing transformed host cells obtained in step (b) in a suitable media to provide for expression of said thermostable DNA polymerase;
- and
- (d) recovering expressed thermostable DNA polymerase of SEQ ID NO. 1 from said cultured transformed host cells.
Dependencies are inferred from the claim text.