Expression vectors and methods of use
AssigneeDana-Farber Cancer Institute, Inc.
InventorsWayne A. Marasco, Jennifer Richardson, Maria Cristina Parolin, Joseph G. Sodroski
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Abstract
The present invention is related to vectors and methods for increasing the expression of a desired gene product. Preferably this invention is used with genes expressing proteins that are not well tolerated by mammalian cells or where high levels of expression are necessary. In certain preferred embodiments it can be used as part of a multi-tiered expression system and with methods of intracellularly targeting a molecule.
Claims9
A lentiviral vector containing a gene of interest operably linked to a selectable marker gene by an internal ribosome entry site (IRES).
The lentiviral vector containing two different genes linked together by an internal ribosome entry site (IRES).
Dependencies are inferred from the claim text.