Chemiluminescent compositions and their use in the detection of hydrogen peroxide

A composition comprising a matrix selected from the group consisting of latex polymers and lipid bilayers, said matrix having incorporated therein a label capable of being activated by singlet oxygen, wherein a peroxidase is bound to the surface of said matrix, said matrix permitting the diffusion of singlet oxygen therein.

A method for detecting hydrogen peroxide or a compound capable of generating hydrogen peroxide, said method comprising:

• (a) providing in combination (i) a sample suspected of containing hydrogen peroxide or said compound and (ii) a composition comprising a matrix having incorporated therein a label capable of being activated by singlet oxygen, wherein a non-photoactivatable catalyst capable of catalyzing the formation of singlet oxygen from hydrogen peroxide is bound to said matrix, said matrix permitting the diffusion of singlet oxygen therein,
• (b) subjecting said combination to conditions wherein said catalyst generates singlet oxygen and
• (c) determining the luminescence produced by reaction of said singlet oxygen with said label, the reaction thereof indicating the presence of said compound.

A method for detecting hydrogen peroxide or a substance capable of generating hydrogen peroxide, said method comprising:

• (a) providing in combination (i) a sample suspected of containing hydrogen peroxide or a substance capable of generating hydrogen peroxide and (ii) a composition comprising a matrix having incorporated therein a label capable of being activated by singlet oxygen, wherein a non-photoactivatable catalyst capable of catalyzing the conversion of hydrogen peroxide to singlet oxygen is bound to the surface of said matrix, said matrix permitting the diffusion of singlet oxygen therein,
• (b) subjecting said combination to conditions wherein hydrogen peroxide reacts with said catalyst to form singlet oxygen and
• (c) determining whether singlet oxygen has reacted with said label, the extent of reaction thereof indicating the presence or amount of hydrogen peroxide or of said substance in said sample.

A method for detecting hydrogen peroxide or a substance capable of generating hydrogen peroxide, said method comprising:

• (a) providing in combination (i) a sample suspected of containing hydrogen peroxide or a substance capable of generating hydrogen peroxide and (ii) a composition comprising a matrix selected from the group consisting of latex polymers and lipid bilayers, said matrix having incorporated therein an olefin capable of reaction with singlet oxygen, wherein a peroxidase is bound to the surface of said matrix, said matrix permitting the diffusion of singlet oxygen therein,
• (b) subjecting said combination to conditions wherein hydrogen peroxide reacts with said peroxidase to form singlet oxygen and
• (c) determining whether singlet oxygen has reacted with said olefin, the reaction thereof indicating the presence of hydrogen peroxide or of said substance in said sample.

A method for detecting an analyte, said analyte being a member of a specific binding pair (sbp), said method comprising:

• (a) providing in combination in a medium (i) a sample suspected of containing said analyte, (ii) an sbp member bound to one of an oxidase or a peroxidase, said sbp member being capable of binding to said analyte or to an sbp member capable of binding to said analyte, (iii) a composition comprising a matrix having incorporated therein a label capable of being modified by singlet oxygen, said matrix permitting the diffusion of singlet oxygen therein, (iv) the other of said oxidase or said peroxidase bound to said matrix or bound to an sbp member capable of binding to said matrix and (v) a substrate for said oxidase capable of generating hydrogen peroxide upon reaction with said oxidase and
• (b) incubating said medium under conditions sufficient to allow said sbp members to bind and said substrate for said oxidase to react with said oxidase and
• (c) determining whether singlet oxygen has reacted with said label, the extent of reaction thereof being related to the presence and/or amount of said analyte in said sample.

A kit comprising in packaged combination:

• (a) a composition comprising a matrix having incorporated therein a label capable of being modified by singlet oxygen, wherein a first enzyme capable of catalyzing the conversion of hydrogen peroxide to singlet oxygen is bound to said matrix or to an sbp member that is capable of binding to said matrix, said matrix permitting the diffusion of singlet oxygen therein, and
• (b) an oxidase.