Sandwich immunoassays for collagen type II degradation products
AssigneeWashington Research Foundation
InventorDavid R. Eyre
Life of this patent
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Abstract
Sandwich immunoassays for detecting analyte indicative of type II collagen resorption in vivo, by contacting a body fluid sample with a first antibody and a second antibody such that analyte present in the sample forms a first antibody-analyte-second antibody complex, and detecting the presence or amount of the first antibody-analyte-second antibody complex, wherein the first and second antibodies are capable of binding to a telopeptide having a sequence identical to that of a carboxy-terminal telopeptide produced in vivo upon degradation of type II collagen.
Claims5
A sandwich immunoassay for detecting analyte indicative of type II collagen resorption in vivo, comprising the steps:
- contacting a nonhydrolyzed body fluid sample with a first antibody and a second antibody such that analyte present in the sample forms a first antibody-analyte-second antibody complex, and
- detecting the presence or amount of the first antibody-analyte-second antibody complex,
- wherein the first and second antibodies are capable of binding to a telopeptide having a sequence identical to that of a carboxy-terminal telopeptide produced in vivo upon degradation of type II collagen.
Dependencies are inferred from the claim text.