Artificial tissues, methods for the production and the use thereof
OwnerMichael Sittinger, Olaf Schultz +2inventors
InventorsMichael Sittinger, Olaf Schultz, Gerd R. Burmester, Thomas E. M. Haupl
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Abstract
The invention relates to new artificial tissues which comprise three-dimensional extracellular matrixes (ECM) in cross-linkable structures, cell interaction systems for inducing artificial three-dimensional tissues and which comprise genetically manipulated cells releasing immunosuppressive or cell-differentiating factors. The tissues according to the invention are suitable for producing vital transplants and for establishing models of diseases.
Claims12
A method of producing new artificial tissues using cell interaction systems, comprising the steps of:
- preparing a first cell culture system, comprising the steps of:
- suspending cells chosen from one or more of the group consisting of differentiated, dedifferentiated and undifferentiated cells, to form a suspension in a solution which can be induced to polymerize,
- applying factors or components which promote the process of differentiation and tissue formation of said cells, and
- adding an inducer of polymerization to effect solidification thereof,
- preparing at least one second cell culture system, comprising the steps of:
- suspending a means for inducing differentiation of the cells of the first cell culture system, said means consisting of one or more chosen from the group consisting of differentiated cells, cells production factors of differentiation and genetically altered cells, to form a suspension in a solution which can be induced to polymerize, and
- adding an inducer of polymerization to effect solidification thereof;
- distributing, prior to the step of adding an inducer of polymerization, either one or both of the suspension of the first cell culture system and the suspension of the second cell culture system within an absorbable fleece;
- and
- contacting said first cell culture system with said second cell culture system
- wherein a new artificial tissue is produced.
Dependencies are inferred from the claim text.