Nucleic acids and methods for detecting pathogenic xanthomonas campestris using the same
AssigneeJapan Tobacco Inc.
InventorTomoki Nishino
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Abstract
The present invention is drawn to nucleic acid fragments specific to Xanthomonas campestris pathogenic to plants belonging to the family Gramineae, as well as methods for detecting the pathogenic Xanthomonas campestris using the same. The nucleic acid fragment of the invention has a nucleotide sequence which is at least 15 consecutive nucleotides of the nucleotide sequence shown in SEQ ID NO:1 or in the complementary chain thereof, or has no less than 15 nucleotides and hybridizes with the nucleic acid having the sequence shown in SEQ ID NO:1 or with the complementary chain thereof under stringent conditions.
Claims16
A nucleic acid fragment consisting of SEQ ID NO:1 or the complement of this sequence or a fragment of at least 15 consecutive nucleotides of SEQ ID NO:1 which specifically hybridizes to the complement of SEQ ID NO:1.
A probe comprising the nucleic acid fragment consisting of SEQ ID NO:1 or the complement of this sequence or a fragment of at least 15 consecutive nucleotides of SEQ ID NO:1 or at least 20 consecutive nucleotides of SEQ ID NO:1 wherein the probe specifically hybridizes to SEQ ID NO:1 or the complement of SEQ ID NO:1.
A method for detecting a Xanthomonas campestris pathogenic to plants belonging to the family Gramineae comprising contacting a nucleic acid sample with a nucleic acid fragment or probe consisting of SEQ ID NO:1 or the complement of this sequence or a fragment of at least 15 consecutive nucleotides of SEQ ID NO:1 which specifically hybridizes to the complement of SEQ ID NO:1 under stringent conditions and detecting a hybridization complex wherein the presence of a hybridization complex is indicative of the presence of Xanthomonas campestris pathogenic to plants belonging to the family Gramineae.
A method for detecting a Xanthomonas campestris pathogenic to plants belonging to the family Gramineae comprising amplifying a nucleic acid having the sequence of SEQ ID NO:1 or the complement thereof, or a fragment thereof specific to Xanthomonas campestris pathogenic to plants belonging to the family Gramineae, and detecting the amplified nucleic acid.
Dependencies are inferred from the claim text.