Antimicrobial peptide
AssigneeHuman Genome Sciences, Inc.
InventorsHenrik S. Olsen, Steven M. Ruben
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Abstract
The present invention relates to a novel human antimicrobial peptide which is a member of the defensin superfamily. In particular, isolated nucleic acid molecules are provided encoding the human antimicrobial peptide. Antimicrobial peptide are also provided as are vectors, host cells and recombinant methods for producing the same. Also provided are diagnostic methods for detecting disorders related to the immune system and therapeutic methods for such disorders.
Claims122
An isolated polynucleotide comprising a nucleic acid at least 95% identical to a reference nucleic acid encoding amino acids 1 to 41 of SEQ ID NO:2, wherein % identity is determined using the Bestfit program with parameters that calculate % identity over the full length of said reference nucleic acid and that allow gaps of up to 5% of the total number of nucleotides of said reference nucleic acid.
An isolated polynucleotide comprising a nucleic acid at least 95% identical to a reference nucleic acid encoding the mature amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97982, wherein % identity is determined using the Bestfit program with parameters that calculate % identity over the full length of said reference nucleic acid and that allow gaps of up to 5% of the total number of nucleotides of said reference nucleic acid.
An isolated polynucleotide comprising a nucleic acid encoding an amino acid sequence at least 95% identical to amino acids 1 to 41 of SEQ ID NO:2 (the reference sequence), wherein % identity is determined using the Bestfit program with parameters that calculate % identity over the full length of said reference sequence and that allow gaps of up to 5% of the total number of residues of said reference sequence.
An isolated polynucleotide comprising a nucleic acid encoding an amino acid sequence at least 95% identical to the mature amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97982 (the reference sequence), wherein % identity is determined using the Bestfit program with parameters that calculate % identity over the full length of said reference sequence and that allow gaps of up to 5% of the total number of residues of said reference sequence.
An isolated polynucleotide comprising a nucleic acid encoding a fragment of SEQ ID NO:2, wherein said fragment exhibits antimicrobial activity.
An isolated polynucleotide comprising a nucleic acid encoding a fragment of the amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97982, wherein said fragment exhibits antimicrobial activity.
An isolated polynucleotide comprising a nucleic acid encoding the mature amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 97982.
An isolated polynucleotide comprising a nucleic acid encoding amino acids 1 to 41 of SEQ ID NO:2.
An isolated polynucleotide comprising a nucleic acid selected from the group consisting of:
- (a) a nucleic acid encoding amino acids 19 to 27 of SEQ ID NO:2;
- and
- (b) a nucleic acid encoding amino acids 31 to 41 of SEQ ID NO:2.
An isolated nucleic acid molecule comprising a first polynucleotide which hybridizes (i) at 42.degree. C. in 50% formamide, 5.times. SSC, 50 mM sodium phosphate, 5.times. Denhardt's solution, 10% dextran sulfate, and 20 .mu.g/ml denatured, sheared salmon sperm DNA;
- (ii) in a wash solution consisting of 0.1.times. SSC at 65.degree. C.;
- to a second polynucleotide selected from the group consisting of:
- (a) a polynucleotide having the sequence of the coding region of the cDNA clone contained in ATCC Deposit No. 97982 or the complement thereof;
- and
- (b) a polynucleotide having the sequence of the coding region of SEQ ID NO:
- 1 or the complement thereof.
An isolated polynucleotide comprising a nucleic acid encoding 30 or more contiguous amino acids of SEQ ID NO:2.
An isolated polynucleotide comprising 30 contiguous nucleotides of the coding region of SEQ ID NO:1.
Dependencies are inferred from the claim text.