HCMV-specific peptides, agents therefor and the use thereof
AssigneeDade Behring Marburg GmbH
InventorsWerner Stuber, Leszek Wieczorek, Robert Ziegelmaier
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Abstract
The invention relates to peptides for the immunochemical determination of human cytomegalovirus (HCMV)-specific antibodies and HCMV antigens, and to agents suitable for this method and to the use thereof.
Claims58
A support-bound peptide which reacts specifically with antibodies against HCMV protein pp 150, consisting of at least one sequence selected from the group consisting of Peptide 1, Peptide 2, and Peptide 3, wherein
- Peptide 1 consists of SEQ ID NO:1 (gly ala gly ala ala ile leu) with
- 0 to 14 additional N-terminal amino acids and 0-7 additional C-terminal amino acids, or
- 0 to 2 additional N-terminal amino acids and 0 to 19 additional C-terminal amino acids;
- Peptide 2 consists of SEQ ID NO:2 (arg ala trp ala leu) with
- 0 to 18 additional N-terminal amino acids and 0 to 18 additional C-terminal amino acids;
- and
- Peptide 3 consists of SEQ ID NO:3 ( ala ser arg asp ala ala) with
- 0 to 11 additional N-terminal amino acids and 0 to 11 additional C-terminal amino acids;
- wherein said additional amino acids comprise contiguous amino acids of HCMV protein pp150.
An immunochemical method for the detection or for the determination of HCMV antibody comprising:
- 1) contacting a sample with at least one support-bound peptide which reacts specifically with antibodies against HCMV protein pp150, consisting of at least one sequence selected from the group consisting of Peptide 1, Peptide 2, and Peptide 3, wherein
- Peptide 1 consists of SEQ ID NO:1 (gly ala gly ala ala ile leu) with
- 0 to 14 additional N-terminal amino acids and 0-7 additional C-terminal amino acids, or
- 0 to 2 additional N-terminal amino acids and 0 to 19 additional C-terminal amino acids;
- Peptide 2 consists of SEQ ID NO:2 (arg ala trp ala leu) with
- 0 to 18 additional N-terminal amino acids and 0 to 18 additional C-terminal amino acids;
- and
- Peptide 3 consists of SEQ ID NO:3 ( ala ser arg asp ala ala) with
- 0 to 11 additional N-terminal amino acids and 0 to 11 additional C-terminal amino acids;
- wherein said additional amino acids comprise contiguous amino acids of HCMV protein pp150;
- 2) forming a peptide-antibody complex;
- and
- 3) detecting or determining said peptide-antibody complex.
An immunochemical combination method for the detection or for the determination of a plurality of antibody specificities against different pathogens, comprising:
- 1) contacting a sample with
- (a) one or more immunoreactive peptides from pathogens other than HCMV, and with
- (b) one or more of the support-bound peptides which react specifically with antibodies against HCMV protein pp150, wherein said support-bound peptides consist of at least one sequence selected from the group consisting of Peptide 1, Peptide 2, and Peptide 3, wherein
- Peptide 1 consists of SEQ ID NO:1 (gly ala gly ala ala ile leu) with
- 0to 14 additional N-terminal amino acids and 0-7 additional C-terminal amino acids, or
- 0 to 2 additional N-terminal amino acids and 0 to 19 additional C-terminal amino acids;
- Peptide 2 consists of SEQ ID NO:2 (arg ala trp ala leu) with 0 to 18 additional N-terminal amino acids and 0 to 18 additional C-terminal amino acids;
- and
- Peptide 3 consists of SEQ ID NO:3 ( ala ser arg asp ala ala) with
- 0 to 11 additional N-terminal amino acids and 0 to 11 additional C-terminal amino acids;
- wherein said additional amino acids comprise contiguous amino acids of HCMV protein pp150.
- 2) forming at least one type of peptide-antibody complex;
- and
- 3) detecting or determining at least one type of peptide-antibody complex.
Dependencies are inferred from the claim text.