Enhanced yield of podophyllotoxin from natural products through in situ conversion methods
AssigneesThe University of Mississippi, The United States of America as represented by the Secretary of Agriculture
InventorsCamilo Canel, Franck E. Dayan, Rita M. Moraes, Charles L. Burandt
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Abstract
Enhanced recovery of podophyllotoxin from biological sources is achieved through disruption or permeabilization of the physical integrity of cells and tissues in the presence of sufficient moisture so as to allow endogenous enzymes to convert podophyllotoxin glucosides to podophyllotoxin. Extraction of the podophyllotoxin from the plant material is then achieved by use of an organic solvent. Podophyllotoxin is used as an intermediate in the production of the antitumor agent etoposide and its analogues.
Claims5
A method of recovering podophyllotoxin from Podophyllum peltatum comprising co-localizing said plant source's endogenous glucosidases and podophyllotoxin glucosides so as to bring them into reactive contact in the presence of sufficient water and effect the substantial conversion of the podophyllotoxin glucosides to podophyllotoxin, and extracting the podophyllotoxin through use of an organic solvent.
Dependencies are inferred from the claim text.