Tumor regression by adenovirus expression of wild-type p53
AssigneeBoard of Regents, The University of Texas System
InventorsWei-Wei Zhang, Jack A Roth
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Abstract
Described are simplified and efficient methods for preparing recombinant adenovirus using liposome-mediated cotransfection and the direct observation of a cytopathic effect (CPE) in the transfected cells. Also disclosed are compositions and methods involving novel p53 adenovirus constructs, including methods for restoring p53 function and tumor suppression in cells and animals having abnormal p53.
Claims94
A method of inducing cell death in human tumor cells comprising administering directly to a tumor comprised of cells which lack functional p53, an adenovirus vector which does not express functional E1B, wherein the vector further comprises and expresses a DNA sequence encoding wild-type p53, and wherein sufficient wild-type p53 is expressed in the tumor cells to induce cell death resulting in regression of the tumor.
A method of inducing cell death in human tumor cells comprising administering directly to a tumor comprised of cells which lack functional p53, an adenovirus vector which does not express functional E1B, wherein the vector further comprises and expresses a DNA sequence encoding wild-type p53 operably linked to the CMV IE promoter, and wherein sufficient wild-type p53 is expressed in the tumor cells to induce cell death resulting in regression of the tumor.
Dependencies are inferred from the claim text.