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US 6,143,154 AGranted
System for PH-neutral stable electrophoresis gel
AssigneeNovex
InventorsTimothy V. Updyke, Sheldon C. Engelhorn
Life of this patent
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Abstract
A gel and buffer system for gel electrophoresis wherein the gel buffer has an approximately neutral pH.
Claims19
A discontinuous buffer gel electrophoresis system comprising:
- an electrophoresis polyacrylamide gel suitable for casting, the polyacrylamide gel comprising a separating gel saturated with a gel buffer comprising an organic amine with a pK.sub.a near neutrality and an anionic compound selected from the group consisting of acetate, bromide, cacodylate, chloride, formate, nitrate, lactate, phosphate, propionate, sulfate, the gel buffer having a pH between 5.5 and 7.5;
- and
- a cathode buffer comprising an antioxidant that migrates into the polyacrylamide gel by electrophoresis, the concentration of the antioxidant sufficient to maintain proteins in a reduced state.
A discontinuous buffer gel electrophoresis system comprising:
- an electrophoresis polyacrylamide gel suitable for casting, the polyacrylamide gel comprising a separating gel saturated with a gel buffer comprising an organic amine with a pK.sub.a near neutrality and an anionic compound selected from the group consisting of acetate, bromide, cacodylate, chloride, formate, nitrate, lactate, phosphate, propionate, sulfate, the gel buffer having a pH between 5.5 and 7.5;
- and
- a cathode buffer comprising an antioxidant that is anionic at neutral pH in a concentration sufficient to maintain proteins in a reduced state.
A method for performing electrophoresis using a discontinuous buffer gel, the method comprising:
- providing an electrophoresis gel suitable for casting, the electrophoresis gel comprising a separating gel;
- saturating the separating gel with a gel buffer comprising an organic amine with a pK.sub.a near neutrality and an anionic compound selected from the group consisting of acetate, bromide, cacodylate, chloride, formate, nitrate, lactate, phosphate, propionate, sulfate, the gel buffer having a pH between 5.5 and 7.5;
- and
- providing a cathode buffer comprising an antioxidant that migrates into the electrophoresis gel by electrophoresis, the concentration of the antioxidant sufficient to maintain proteins in a reduced state.
1 more element
Dependencies are inferred from the claim text.