Back to results
US 6,143,152 AGranted
Microfabricated capillary array electrophoresis device and method
AssigneeThe Regents of the University of California
InventorsPeter C. Simpson, Richard A. Mathies, Adam T. Woolley
Life of this patent
Estimating the term…
Abstract
A capillary array electrophoresis (CAE) micro-plate with an array of separation channels connected to an array of sample reservoirs on the plate. The sample reservoirs are organized into one or more sample injectors. One or more waste reservoirs are provided to collect wastes from reservoirs in each of the sample injectors. Additionally, a cathode reservoir is also multiplexed with one or more separation channels. To complete the electrical path, an anode reservoir which is common to some or all separation channels is also provided on the micro-plate. Moreover, the channel layout keeps the distance from the anode to each of the cathodes approximately constant.
Claims41
A capillary array electrophoresis plate, comprising:
- one or more anode reservoirs;
- a plurality of separation channels connected to said anode reservoirs;
- and
- one or more injectors formed in said plate, each injector including:
- two or more sample reservoirs coupled to said separation channels;
- a plurality of waste reservoirs connected to said separation channels;
- and
- at least one cathode reservoir multiplexed with two or more of said separation channels.
5 more elements
A capillary array electrophoresis plate comprising:
- an array of microfabricated separation channels formed at a surface of a first microfabricated substrate and a corresponding surface of a second substrate bonded to the surface of said first substrate, each of said channels including a first end and a second end;
- an array of sample reservoirs connected to said array of separation channels;
- an array of waste reservoirs connected to said array of separation channels;
- an array of cathode reservoirs connected to the first end of each of the separation channels;
- an array of anode reservoirs, wherein at least one anode reservoir is connected to the respective second ends of at least two of the separation channels;
- and
- an injector formed by an injection channel connected to a sample reservoir that crosses a separation channel and connects to a waste reservoir.
4 more elements
A capillary array electrophoresis plate comprising:
- an array of microfabricated separation channels formed on a surface of a first microfabricated substrate and a corresponding surface of a second substrate bonded to the surface of said first substrate, each of said channels having a first end and a second end;
- an array of sample reservoirs connected to said array of separation channels;
- an array of waste reservoirs connected to said array of separation channels;
- an array of anode reservoirs connected to the second end of each of the separation channels;
- an array of cathode reservoirs, wherein at least one cathode reservoir is connected to the respective first ends of at least two of the separation channels;
- and
- an injector formed by an injection channel crossing one of said separation channels, said injection channel connecting one sample reservoir of said array of sample reservoirs to one waste reservoir of said array of waste reservoirs.
4 more elements
A capillary array electrophoresis plate comprising:
- an array of microfabricated separation channels formed on a surface of a first microfabricated substrate and a corresponding surface of a second substrate bonded to the surface of said first substrate, each of said channels having a first end and a second end;
- an array of sample reservoirs connected to said array of separation channels wherein each separation channel of the array of separation channels is in fluid communication with at least one dedicated sample reservoir;
- and
- an array of waste reservoirs connected to said separation channels, wherein at least one waste reservoir is multiplexed to two or more of said channels.
1 more element
A capillary array electrophoresis plate, comprising:
- a plurality of separation channels formed in said plate;
- a plurality of sample reservoirs connected to said separation channels wherein each separation channel of the plurality of separation channels is in fluid communication with at least one dedicated sample reservoir;
- and
- an anode reservoir multiplexed to two or more of the plurality of separation channels.
1 more element
A capillary electrophoresis plate, comprising:
- a plurality of separation channels formed in said plate;
- a plurality of sample reservoirs coupled to said separation channels wherein each separation channel of the plurality of separation channels is in fluid communication with at least one dedicated sample reservoir;
- and
- a cathode reservoir multiplexed to two or more of the plurality of separation channels.
1 more element
A capillary array electrophoresis plate, comprising:
- a plurality of separation channels formed in said plate;
- a plurality of sample reservoirs coupled to said separation channels wherein each separation channel of the plurality of separation channels is in fluid communication with at least one dedicated sample reservoir;
- and
- a waste reservoir multiplexed to two or more of the plurality of separation channels.
1 more element
A method for injecting a sample through a capillary array electrophoresis plate, comprising:
- connecting a cathode reservoir to respective first ends of two or more separation channels;
- connecting an anode reservoir to respective second ends of two or more of said separation channels;
- connecting a sample reservoir and a waste reservoir together by a cross channel that crosses one of said separation channels;
- applying an injection voltage between the sample reservoir and the waste reservoir to draw the sample into the cross channel while applying a bias voltage to the cathode reservoir and the anode reservoir to control an injection plug width;
- applying a running voltage between the cathode and anode reservoirs;
- and
- applying a biasing voltage to the waste reservoir and the sample reservoir to pull away residuals of the sample.
4 more elements
A method of forming a capillary array electrophoresis plate, comprising:
- forming a plurality of separation channels in said plate;
- forming a plurality of sample reservoirs connected to said separation channels wherein each separation channel of the plurality of separation channels is in fluid communication with at least one dedicated sample reservoir;
- and
- connecting an anode reservoir to two or more of the plurality of separation channels.
1 more element
A method of forming a capillary array electrophoresis plate, comprising:
- forming a plurality of separation channels in said plate;
- forming a plurality of sample reservoirs connected to said separation channels wherein each separation channel of the plurality of separation channels is in fluid communication with at least one dedicated sample reservoir;
- and
- connecting a cathode reservoir to two or more of the plurality of separation channels.
1 more element
A method of forming a capillary array electrophoresis plate, comprising:
- forming a plurality of separation channels in said plate;
- forming a plurality of sample reservoirs connected to said separation channels wherein each separation channel of the plurality of separation channels is in fluid communication with at least one dedicated sample reservoir;
- and
- connecting a waste reservoir to two or more of the plurality of sample reservoirs.
1 more element
A method of forming a capillary array electrophoresis plate, comprising:
- forming an array of microfabricated separation channels;
- forming an array of sample reservoirs;
- connecting the array of sample reservoirs to the array of microfabricated separation channels;
- connecting one or more cathode reservoirs to said array of separation channels, wherein at least one cathode reservoir is connected to two or more separation channels of said array of separation channels;
- connecting one or more anode reservoirs to said array of separation channels, wherein at least one anode reservoir is connected to two or more separation channels of said array of separation channels;
- and
- connecting an array of waste reservoirs to said array of separation channels, wherein at least one waste reservoir of said array of waste reservoirs is connected to two or more sample reservoirs of said array of sample reservoirs.
4 more elements
A capillary array electrophoresis plate, comprising:
- a first set of cathode reservoirs positioned near an outer perimeter of the plate;
- a second set of anode reservoirs positioned near a center of the plate, wherein the number of reservoirs in said second set is fewer than the number of reservoirs in said first set;
- and
- an array of separation channels connecting the first set of reservoirs to the second set of reservoirs.
1 more element
A capillary array electrophoresis plate, comprising:
- a separation channel formed in said plate connected to a cathode reservoir and an anode reservoir;
- a first cross channel connected to the separation channel at a first location, wherein a first end of the first cross channel connects to a first waste reservoir and a second end connects to a first sample reservoir;
- and
- a second cross channel connected to the separation channel at a second location;
- wherein a first end of the second cross channel connects to the first waste reservoir and a second end connects to a second sample reservoir.
2 more elements
A capillary array electrophoresis plate comprising:
- an array of microfabricated separation channels formed on a surface of a first microfabricated substrate and a corresponding surface of a second substrate bonded to the surface of said first substrate, each of said channels having a first end and a second end;
- an array of sample reservoirs connected to said array of separation channels wherein each of a plurality of individual separation channels are each in fluid communication with at least one dedicated sample reservoir;
- and an array of waste reservoirs connected to said separation channels, wherein at least one waste reservoir is multiplexed to two or more of said channels, wherein the capillary array electrophoresis plate has H holes, and wherein H is approximately equal to 5N/4, with N being the number of samples to be processed.
A capillary array electrophoresis plate comprising:
- a microfabricated separation channel formed on a surface of a first microfabricated substrate and a corresponding surface of a second substrate bonded to the surface of said first substrate, said channel being connected to a cathode reservoir on one end and an anode reservoir on the other end;
- and two or more sample reservoirs sharing a waste reservoir and connected to the separation channel, wherein the capillary array electrophoresis plate has H holes, and wherein H is approximately equal to 5N/4, with N being the number of samples to be processed.
Dependencies are inferred from the claim text.