Wheel information estimating apparatus

A wheel method of assaying translational recoding in vitro comprising

• (a) providing a translational reporter vector comprising a polylinker interposed between first and second luciferase coding sequences wherein said first and second luciferase coding sequences are in different reading frames;
• (b) inserting a DNA to be tested in said reporter vector at the polylinker to form a test vector such that the first and second luciferase coding sequences remain in different reading frames;
• (c) inserting the DNA to be tested in said reporter vector at the polylinker to form a control vector such that the first and second luciferase coding sequences are in the same reading frame;
• (d) separately transcribing said reporter vector, said test vector, and said control vector in vitro to result in respective RNAs comprising transcription copies of said first luciferase coding sequence said DNA to be tested, and said second luciferase coding sequence;
• (e) separating translating each of said RNAs in vitro to result in translation products thereof;
• (f) determining luminescences attributable to each of said translation products, wherein the tranaslation products of the reporter vector contributed background luminescence, the translation products of the control vector contribute normal luminescence, and the translation products of the test vector contribute test luminescence;
• (g) subtracting background luminescence from said normal and said test luminescences and normalizing said test luminescence wiht respect to said normal luminescence.