US 6,140,096 A · Enzyme with endo-1,3(4)-.beta.-glucanase activity

Abstract

An enzyme having endo-1,3(4)-.beta.-glucanase activity is described which is encoded by the DNA sequence ATGTGGTCTCCCAAGGTTGCTGCTGCCGTCCTCGCCTTTGTTGGTGCTACCAACGCCT GGCAGCCCCCGACCTACAGCGGCTTCAACTTGGTCTGGACTGACACCTTCGCTGGCAACGGTGGCACTTCTCCT A ACCAGAACAACTGGAACATCATCACCGGAAACTTGAACGTCAACGCCGAGCAGGAGACCTACTCCTCCAGCAC C GCCAATGTTCAGCTCAGTGGTGGCAGCACCCTTCAGCTGGTCCCCTGGAGAGACAGCAGCAAGGGAACCAGCA C CTTTGGTGGCTGGACCTCCGGTCGTCTTGAGTCCAAGTACACATTCACTCCCGCGGCCGGCAAGGTCACCCGT CTT GAAGCCGCCATCCGCTTCGGCAGCAACGCTCAGGCCAACAAGCAGGGTATCTGGCCTGCTTTCTGGATGCTGGG T GACTCCCTCCGTCAACCGGGCGGCAGCTGGCCCAACTGTGGTGAGATCGACATCATGGAGACTGTCGACGGCC A GGCTACCGGCCACGGTACCCTTCACTGCGACGTCTACCCCGGCGGTATCTGCAACGAGGGTAACGGTATTGGA GG CCCTGTCAACATCGCCAACGTCAACGACTGGCACGCTTGGCGTGTTGAGATCGACCGCACTCCCAGCAGCTGGC A ATCCGAGACCCTCACCTGGTCCCTCGACGGCACCATCTACTTCCAGATCACTGGCTCTCGCATTGGCAACCAG GG CGTCTGGAACAACATTGCTCACAGCCCCCTCTTCTTCATTCTTAACGTTGCTGTCGGTGGCAACTGGCCTGGCA AC CCCAACAGCGCTACCCTCGATGGCTACGGAAGCATGATGGAGGTTGGCTACGTCGCTCAGTACTCTACCTAA (SEQ ID NO:3).

Claims22

Dependent claims

A DNA construct comprising a DNA sequence encoding an enzyme exhibiting endo-.beta.-glucanase activity, wherein the DNA sequence is one of:

(a) a DNA sequence comprising the sequence of SEQ ID NO:3,
(b) a DNA sequence which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:4, or
(c) a DNA sequence which hybridizes with the oligonucleotide probe comprising the DNA sequence of (a) under conditions of presoaking in 5.times.SSC, prehybridizing for 1 hr at .about.50.degree. C. in a solution of 5.times.SSC, 5.times.Denhardt's, 50 mM sodium phosphate, pH 6.8, and 50 .mu.g denatured sonicated calf thymus DNA, followed by hybridization in tlle same solution for 18 h at .about.50.degree. C., followed by washing three times in 2.times.SSC, 0.2% SDS at 50.degree. C. for 30 minutes.

Dependencies are inferred from the claim text.

Enzyme with endo-1,3(4)-.beta.-glucanase activity

Life of this patent

Enzyme with endo-1,3(4)-.beta.-glucanase activity

Life of this patent

Abstract

Claims22