Protein with phospholipase activity
AssigneeRohm Co., Ltd.
InventorsFridolin Loffler, Gerald Jungschaffer, Quoc Nguyen Khanh, Erwin Schuster, Bruno Sprossler, Sabine Wolf
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AssigneeRohm Co., Ltd.
InventorsFridolin Loffler, Gerald Jungschaffer, Quoc Nguyen Khanh, Erwin Schuster, Bruno Sprossler, Sabine Wolf
Estimating the term…
This invention relates to a protein having phospholipase activity, which is characterised in that it has the mature sequence of Aspergillus lysophospholipase or a sequence derived therefrom and that it may be cleaved at at least one site, wherein, in the event of cleavage, the restriction fragments are optionally either linked by means of at least one bond cleavable under reducing conditions or at least one of the unlinked restriction fragments has phospholipase activity, and to a process for the production of this protein by fermenting a suitably transformed lysophospholipase-producing host organism in a suitable culture medium and isolating the protein having phospholipase activity from the cell-free culture filtrate, wherein fermentation is performed in the acidic to slightly alkaline range.
An isolated two-chain phospholipase cleavage fragment having phospholipase activity but does not possess lysophospholipase activity, wherein the phospholipase cleavage fragment is obtained by cleaving a mature sequence of Aspergillus lysophospholipase of SEQ ID NO:2 into two cleavage fragments that are linked by at least one bond that is cleavable under reducing conditions.
Dependencies are inferred from the claim text.