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AssigneeUnilever Patent Holdings B.V.
InventorsJan W. Sanders, Jan Kok, Gerard Venema, Adrianus M Ledeboer
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The invention provides a salt-inducible promoter present in SEQ ID NO: 10 and derivable from a lactic acid bacterium in isolation from the coding sequence normally controlled by said promoter in a wild-type lactic acid bacterium, with modifications and important parts thereof. Also provided are a recombinant vector and a transformed lactic acid bacterium comprising such promoter, and the production of a desired protein by such transformed bacterium, whereby the gene encoding said desired protein or a precursor thereof is expressed under control of such promoter. The desired protein can be secreted by the bacterium due to the presence of a signal sequence. The action of the salt-inducible promoter is enhanced at a pH of about 4-4.5 and/or by the presence of glutamic acid. Such process can be used in a fermentation process, in which the desired protein is a lytic protein causing lysis of the cells and release of the cell content. Or the desired protein can be an enzyme involved in flavour formation, e.g. in producing dressings, spreads, sausages and sour dough, or a protein functioning in a cheese production process, such as chymosin or a precursor thereof, or an enzyme involved in cheese flavour formation.
A salt-inducible promoter obtained from a lactic acid bacterium, in isolation from the coding sequence which is normally controlled by said promoter in a wild-type lactic acid bacterium.
A salt-inducible promoter, which exerts promoter activity for salt-induction of gene expression and comprises a DNA sequence that hybridizes with a polynucleotide selected from the group consisting of
A DNA fragment encoding a polypeptide that regulates a salt-inducible promoter active in a lactic acid bacterium, which comprises the polynucleotide 1095-1925 of SEQ. ID. NO:
A recombinant vector comprising a salt-inducible promoter selected from the group consisting of:
A transformed lactic acid bacterium comprising a salt-inducible promoter from a lactic acid bacterium, in isolation from the coding sequences which is normally controlled by said promoter in a wild-type lactic acid bacterium;
Dependencies are inferred from the claim text.