Preparation of cell seeded ceramic compositions
AssigneeEtex Corporation
InventorsDosuk D. Lee, Christian Rey, Maria Aiolova
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Abstract
The present invention provides a synthetic, poorly-crystalline apatitic (PCA) calcium phosphate material seeded with cells. Preferably, the cells are tissue-forming or tissue-degrading cells. The compositions provided by the present invention are useful for a variety of applications, including in vivo and in vitro tissue growth (preferably bone or cartilage), osseous augmentation, and methods of diagnosing disease states by assaying tissue-forming potential of cells isolated from a host. The invention also provides in vitro cell culture systems and cell encapsulation matrices.
Claims30
A method for preparing a therapeutic, structural or cosmetic implant, comprising:
- a. providing a composition in hydrated precursor form, wherein the hydrated precursor is capable of conversion into a hardened poorly crystalline apatitic (PCA) calcium phosphate;
- b. promoting conversion of the hydrated precursor so that the composition becomes hardened PCA calcium phosphate;
- and
- c. introducing at least one cell into the composition prior to hardening of the hydrated precursor.
A method for preparing a structural implant comprising:
- a. providing a composition in hydrated precursor form, wherein the hydrated precursor is capable of conversion into a hardened poorly crystalline apatitic (PCA) calcium phosphate;
- b. promoting conversion of the hydrated precursor so that the composition becomes hardened PCA calcium phosphate, said PCA calcium phosphate composition having sufficient tensile strength, hardness and fracture toughness so that said PCA calcium phosphate composition is capable of serving as a structural implant in a living organism;
- and
- c. before or after step b, introducing at least one cell into the composition.
Dependencies are inferred from the claim text.