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US 6,136,959 AGranted
High efficiency alkaline protein extraction
AssigneeUniversity of Massachusetts
InventorsHerbert O. Hultin, Stephen D. Kelleher
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Abstract
A process for isolating edible protein from animal muscle by solubilizing the protein in an alkaline aqueous solution is disclosed.
Claims29
A method for isolating edible protein from animal muscle, the method comprising:
- (a) obtaining a mixture comprising animal muscle and water, wherein the animal muscle comprises less than about 15% by weight of the mixture;
- (b) increasing the pH of the mixture to a level sufficient to solubilize at least 75% of the animal protein in the animal muscle mixture;
- (c) centrifuging the mixture, after at least 75% of the animal protein is solubilized, at about 5,000.times.g or higher so that membrane lipids are separated from an aqueous phase, and collecting the aqueous phase;
- (d) precipitating the protein from the aqueous phase;
- and
- (e) collecting the precipitated protein, thereby isolating the edible protein from the animal muscle, wherein the collected precipitated protein provides a yield of at least 70% of the total animal muscle protein.
3 more elements
A method for isolating edible protein from animal muscle, the method comprising:
- (a) obtaining animal muscle;
- (b) adding an aqueous solution to the animal muscle to form a mixture such that the animal muscle comprises less than about 15% by weight of the mixture, wherein the pH of the aqueous solution is sufficiently alkaline to solubilize at least 75% of the animal protein in the animal muscle mixture;
- (c) centrifuging the mixture, after at least 75% of the animal muscle protein is solubilized, at about 5000.times.g or higher so that membrane lipids are separated from an aqueous phase, and collecting the aqueous phase;
- (d) precipitating the protein from the aqueous phase;
- and
- (e) collecting the precipitated protein, thereby isolating the edible protein from the animal muscle, wherein the collected precipitated protein provides a yield of at least 70% of the total animal muscle protein.
3 more elements
Dependencies are inferred from the claim text.