Method of inactivating lipid-enveloped viruses
AssigneeBaxter Aktiengesellschaft
InventorsNoel Barrett, Otfried Kistner, Friedrich Dorner
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Abstract
The invention relates to a method of inactivating lipid-enveloped viruses by means of a non-ionic detergent, and the preparation of a vaccine containing the inactivated virus. The invention further relates to an inactivated virus which is characterized by its structural integrity, in particular the structural integrity of its enveloping proteins, as well as to the use of the inactivated virus for preparing a vaccine.
Claims16
A method of inactivating lipid-enveloped viruses comprising
- incubating (i) a whole lipid-enveloped virus having envelope proteins with (ii) a polysorbate selected from the group consisting of Tween.RTM. 80, Tween.RTM. 40, Tween.RTM. 20, and Tween.RTM. 60 at a concentration between 1% to 20% for a period of time sufficient to inactivate said lipid-enveloped virus without disintegrating said whole virus, and without destroying the envelope proteins.
A method of preparing an inactivated whole virus, comprising
- providing an inactivated whole lipid-enveloped virus having envelope proteins, said virus having been inactivated by incubating a whole virus with a polysorbate selected from the group consisting of Tween.RTM. 80, Tween.RTM. 40, Tween.RTM. 20, and Tween.RTM. 60 at a concentration between 1% to 20% for a period of time sufficient to inactivate said virus without disintegrating said whole virus, and without destroying the envelope proteins, and
- admixing said inactivated lipid-enveloped virus with a physiologically acceptable carrier.
A virus preparation containing inactivated whole lipid-enveloped viruses, wherein the inactivated whole viruses comprise viral envelope proteins that are intact and possess native form and structure.
A method of inducing an immune response in a vertebrate comprising administering to the vertebrate a virus preparation containing inactivated whole lipid-enveloped viruses, wherein the inactivated whole lipid-enveloped viruses have been inactivated by incubating a whole lipid-enveloped virus with a polysorbate detergent selected from the group consisting of Tween.RTM. 80, Tween.RTM. 20, Tween.RTM. 60, and Tween.RTM. 40 at a concentration between 1% to 20% for a period of time sufficient to inactivate said virus without disintegrating said whole virus.
Dependencies are inferred from the claim text.