Superparamagnetic monodisperse particles
AssigneeBio Merieux
InventorsAbdelhamid Elaissari, Christian Pichot, Bernard Mandrand, Florence Sauzedde
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Abstract
The superparamagnetic monodispersed particles comprise a core of a first polymer, an internal layer of a second polymer coating the core and in which a magnetic material is distributed, and an external layer of a third polymer coating the magnetic layer and capable of interacting with at least one biological molecule. At least the second polymer is heat sensitive and has a predetermined lower critical solubility temperature (LCST) of 15-65.degree. C. These particles may be used to isolate at least one biological molecule from a liquid specimen.
Claims23
Monodisperse superparamagnetic particles having a predetermined size of between 0.1 and 10 .mu.m, comprising:
- a core based on a first polymer,
- an internal layer, called the magnetic layer, covering the core, based on a second polymer, wherein in said internal layer a magnetic material is distributed, and
- an external layer, called the encapsulation layer, optionally functionalized, covering the magnetic layer, based on a third polymer and having an ability to interact with at least one biological molecule to fix the at least one biological molecule to the particles, either directly or indirectly,
- wherein at least the second polymer is heat-sensitive and has a predetermined lower critical solubility temperature (LCST) of between 15 and 65.degree. C.
Dependencies are inferred from the claim text.