Methods and compositions related to the production of trehalose
AssigneeCalgene, Inc.
InventorsArne Reidar Strom, Inga Kaasen, Olaf Bay Styrvold, John McDougall
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Abstract
This invention relates to genes involved in the biosynthesis of trehalose. The genes encode trehalose-6-phosphate synthase (trehalose synthase) and trehalose-6-phosphate phosphatase (trehalose phosphatase).
Claims19
A purified recombinant construct comprising a DNA sequence encoding a bacterial trehalose biosynthetic enzyme selected from the group consisting of trehalose synthase and trehalose phosphatase.
A chimeric gene comprising, in the 5' to 3' direction of transcription, the following genetic elements linked in operable combination:
- (i) a transcription initiation region functional in a plant cell;
- (ii) a translation initiation region functional in a plant cell;
- (iii) a DNA sequence encoding a trehalose biosynthetic enzyme selected from the group consisting of bacterial trehalose synthase and bacterial trehalose phosphatase;
- and
- (iv) a translation termination region functional in a plant cell.
A method of producing an increased amount of a trehalose biosynthetic enzyme in a host cell as compared with a wild type host cell normally capable of producing said enzyme, said method comprising:
- inserting a DNA sequence into a host cell, said sequence encoding a trehalose biosynthetic enzyme selected from the group consisting of bacterial trehalose synthase and bacterial trehalose phosphatase, operably linked to regulatory elements for directing the expression of said enzyme in said host cell;
- and
- growing said host cell under conditions to permit the expression of said enzyme.
A method of producing a host cell for increased trehalose production comprising the steps of:
- inserting a first DNA sequence into said host cell, said sequence encoding a trehalose synthase operably linked to regulatory elements for directing the expression of said trehalose synthase in said host cell in the presence of UDP-glucose and glucose-6-phosphate, and a second DNA sequence encoding a trehalose phosphatase operably linked to regulatory elements for directing the expression of said trehalose phosphatase in said host cell wherein trehalose phosphatase encoded by said trehalose phosphatase encoding DNA sequence is available to the product of said trehalose synthase in the presence of said UDP-glucose and said glucose-6-phosphate;
- and
- growing said host cell under conditions to permit the expression of said synthase and said phosphatase whereby trehalose is produced.
A method of producing trehalose biosynthetic enzyme in a host cell otherwise incapable of producing said enzyme, said method comprising:
- inserting a DNA sequence into a host cell, said sequence encoding a trehalose biosynthetic enzyme selected from a group consisting of bacterial trehalose synthase and bacterial trehalose phosphatase, operably linked to regulatory elements for directing the expression of said enzyme in said host cell;
- and
- growing said host cell under conditions to permit the expression of said enzyme.
Dependencies are inferred from the claim text.