Co-cultivation of cells in a micropatterned configuration

A method for producing a micropatterned co-culture containing at least two cell types, the method comprising:

• i) providing a substrate coated with a cell-binding protein which defines a micropattern on the substrate;
• ii) contacting the cell-binding protein with cells of a first cell type suspended in a first cell medium under conditions such that cells of the first cell type bind the cell-binding protein, thereby producing a micropatterned cell-coated substrate;
• and
• iii) contacting the micropatterned cell-coated substrate with cells of a second cell type suspended in a second cell medium under conditions such that cells of the second cell type bind the substrate, thereby producing the micropatterned co-culture, wherein one of the cell media is a selective medium that lacks serum and attachment factors and/or includes a non-adhesive factor to inhibit attachment, and one of the cell media is an attachment medium that contains an effective amount of serum and/or at least one attachment factor.