Recombinant vector containing a sequence of a lipoprotein gene for the expression of nucleotide sequences
OwnerRaymond Hamers, Pierre Cornelisinventors
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Abstract
A recombinant vector for cloning a heterologous nucleotide sequence and/or expressing it and/or transferring it to a cell host. The vector includes, at a site which is not essential for replication, the gene coding for a lipoprotein other than E. coli lipoproteins, or a part of said gene which contains the elements required for controlling the expression of said lipoprotein and exposing it on the surface of the outer host cell membrane, so that the heterologous nucleotide sequence can be inserted into the gene or said part thereof under conditions suitable for expressing said heterologous sequence and exposing it on the surface of the cell host.
Claims23
A recombinant vector for the cloning, expression, and exposure at the surface of the external membrane of Gram-negative bacteria of a heterologous polypeptide, said vector comprising a nucleotide sequence encoding a fusion protein consisting of the major lipoprotein of the external membrane of the P. aeruginosa, OprI, and the heteologous polypeptide.
A recombinant Gram-negative bacterium host cell which is transformed with a vector selected from the group consisting of pLPI34 (LMBP2916), pLPI35, pLPI36, pVUB1 and pVUB2.
A recombinant vector for the expression into a host cell and exposure at the surface of the host cell, of a heterologous nucleotide sequence, containing, at a site not essential for its replication, a first gene encoding the major lipoprotein of the external membrane of P. aeruginosa, OprI, and a heterologous nucleotide sequence which is introduced into said first gene at the level of the N-terminal part of the mature OprI lipoprotein, under conditions allowing the expression of this heterologous sequence and its exposure at the surface of the cell host.
A recombinant vector for the expression, into a host cell and exposure at the surface of the cell host, of a heterologous nucleotide sequence, containing, at a site not essential for its replication, a first gene encoding the major lipoprotein of the external membrane of P. aeruginosa, OprI, and a heterologous nucleotide sequence which is introduced into said first gene at the level of the N-terminal part of the mature OprI lipoprotein, 3 or 4 amino acids following the N-terminal end, under conditions allowing the expression of this heterologous sequence and its exposure at the surface of the external membrane of the cell host.
Dependencies are inferred from the claim text.