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US 6,127,173 AGranted
Nucleic acid catalysts with endonuclease activity
AssigneeRibozyme Pharmaceuticals, Inc.
InventorsFritz Eckstein, Paul A. Heaton, Narendra K. Vaish
Life of this patent
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Abstract
Nucleic acid molecules with new motifs having catalytic activity, methods of synthesis, and use thereof are also described.
Claims28
A nucleic acid molecule with an endonuclease activity having the formula I:
- ##STR2## wherein, each N represents independently a nucleotide or an abasic moiety, which may be same or different;
- X and Y are independent oligonucleotides of length sufficient to stably interact with a target nucleic acid molecule;
- m and n are integers greater than or equal to 1, wherein if (N).sub.m and (N).sub.n are nucleotides, (N)m and (N)n may interact by hydrogen bond interaction;
- J is an oligonucleotide having a sequence selected from the group consisting of 5'-GGCAUCC-3', 5'-AGCAUU-3', 5'-GCAUCA-3', 5'-AGCAUC-3', and, 5'-AGCGUC-3';
- L is a linker which may be present or absent, wherein said linker, when present, is a nucleotide linker or a non-nucleotide linker, or a combination of nucleotide and a non-nucleotide linker, wherein said nucleotide linker comprises a single-stranded region, or double-stranded region, or a combination of single-stranded and a double-stranded region;
- represents a chemical linkage;
- and C and G represent cytidine and guanosine nucleotides, respectively.
4 more elements
A nucleic acid molecule with an endonuclease activity having the formula II:
- ##STR3## wherein, each N represents independently a nucleotide or a non-nucleotide linker, which may be same or different;
- X and Y are independent oligonucleotides of length sufficient to stably interact with a target nucleic acid molecule;
- m and n are integers greater than or equal to 1, wherein if (N).sub.m and (N).sub.n are nucleotides, (N)m and (N)n may interact by hydrogen bond interaction;
- J is an oligonucleotide of length 6 nucleotides having a sequence 5'-GGAAUC-3';
- L is a linker which may be present or absent, wherein said linker, when present, is a nucleotide linker or a non-nucleotide linker, or a combination of nucleotide and a non-nucleotide linker, wherein said nucleotide linker comprises a single-stranded region, or double-stranded region, or a combination of single-stranded and a double-stranded region;
- represents a chemical linkage;
- and C represents cytidine nucleotide.
4 more elements
A nucleic acid molecule with endonuclease activity having the formula III:
- ##STR4## wherein, each N represents independently a nucleotide or a non-nucleotide linker, which may be same or different;
- X and Y are independent oligonucleotides of length sufficient to stably interact with a target nucleic acid molecule;
- m and n are integers greater than or equal to 1, wherein if (N).sub.m and (N).sub.n are nucleotides, (N)m and (N)n may interact by hydrogen bond interaction;
- K is an oligonucleotide of length 5 nucleotides having a sequence selected from the group consisting of 5'-UGAUG-3', 5'-UGAUC-3', and 5'-UGGGC-3';
- L is a linker which may be present or absent, wherein said linker, when present, is a nucleotide linker or a non-nucleotide linker, or a combination of nucleotide and a non-nucleotide linker, wherein said nucleotide linker comprises a single-stranded region, or double-stranded region, or a combination of single-stranded and a double-stranded region;
- represents a chemical linkage;
- and C and G represent cytidine and guanosine nucleotides, respectively.
4 more elements
Dependencies are inferred from the claim text.